Changes in cytoplasmic chromatophily and RNA content in neurons of the cingulate cortex of rats with subhepatic cholestasis

Abstract

BACKGROUND: Cholestasis refers to the stagnation of bile, leading to the release of its components into the blood, which has a toxic effect on various organs and systems, including the brain, and is accompanied by cognitive and behavioral disorders. The cingulate cortex is responsible for emotions, learning, and memory. Studies of this area of the brain will help understand the mechanisms of neuropsychiatric disorders in this pathology. Moreover, studying the cytoplasmic chromatophily of cingulate cortex neurons and the RNA content will help elucidate the morphofunctional state and severity of damage to neurons and their protein synthetic apparatus (ribosomes containing RNA).

AIM: To examine the RNA content and cytoplasmic chromatophily of neurons in the cingulate cortex of the rat brain with cholestasis.

MATERIALS AND METHODS: Neurons of the second parvocellular and fifth magnocellular layers of the anterior cingulate cortex of rats were evaluated at different times after ligation/transection of their common bile duct or sham operation (control). Histological, histochemical, morphometric, and statistical analyses were performed.

RESULTS: Structural and histochemical changes in cingulate cortical neurons increase as cholestasis worsens in rats. Moreover, the number of normochromic neurons and shadow cells and hyperchromic, hyperchromic wrinkled, hypochromic neurons, and shadow cells increased, whereas the RNA content in the remaining neurons decreased. These changes reach a maximum of 10–20 days after the transection of the common bile duct. In surviving animals, in the preserved neurons of the cingulate cortex on days 45–90 after surgery, cytoplasmic chromatophily and RNA content are gradually normalized. Disturbances in the morphofunctional state of the neurons during cholestasis begin and end quite earlier in the small-cell than in the large-cell layer of the cingulate cortex.

CONCLUSION: As cholestasis increases among the neurons of the rat cingulate cortex, the number of normochromic cells decreases, whereas the number of neurons with changes in cytoplasmic chromatophily and reduction in the RNA content increases. This indicates severe morphofunctional disorders of neurons and their protein-synthesizing capabilities. As cholestasis is eliminated, the studied parameters gradually normalized in the surviving neurons, which confirms the high adaptive capabilities of rat brain neurons.

Keywords

cholestasis / cingulate cortex of the rat / neurons / chromatophilia / RNA

References

[1] Yuldasheva GB. Prevalence of cholelithiasis and modern treatments. Re-Health Journal. 2022;(1):42–50.

[2] Юлдашева Г.Б. Распространенность желчнокаменной болезни и современные методы лечения // Re-Health Journal. 2022. № 1. С. 42–50.

[3] Yuldasheva GB. Prevalence of cholelithiasis and modern treatments. Re-Health Journal. 2022;(1):42–50.

[4] Esfahani DE, Zarrindast MR. Cholestasis and behavioral disorders. Gastroenterol Hepatol Bed Bench. 2021;14(2):95–107. EDN: CVTEDA doi: 10.22037/ghfbb.v14i2.1971

[5] Esfahani D.E., Zarrindast M.R. Cholestasis and behavioral disorders // Gastroenterol Hepatol Bed Bench. 2021. Vol. 14, N 2. Р. 95–107. EDN: CVTEDA doi: 10.22037/ghfbb.v14i2.1971

[6] Esfahani DE, Zarrindast MR. Cholestasis and behavioral disorders. Gastroenterol Hepatol Bed Bench. 2021;14(2):95–107. EDN: CVTEDA doi: 10.22037/ghfbb.v14i2.1971

[7] Zimatkin SM, Emelyanchik SV. Brain neurons in bile circulation disorders. Grodno: Grodno State Medical University; 2021. 368 p. (In Russ.).

[8] Зиматкин С.М., Емельянчик С.В. Нейроны мозга при нарушениях циркуляции желчи. Гродно: ГрГМУ, 2021. 368 с.

[9] Zimatkin SM, Emelyanchik SV. Brain neurons in bile circulation disorders. Grodno: Grodno State Medical University; 2021. 368 p. (In Russ.).

[10] Mazhirina KG, Pervushina ON, Fedorov AA, et al. Neuroimaging and the phenomenon of moral choice. Uspekhi fiziologicheskikh nauk. 2021;52(1):16–30. EDN: MTXXXZ doi: 10.31857/S0301179821010057

[11] Мажирина К.Г., Первушина О.Н., Федоров А.А., и др. Нейровизуализация и феномен морального выбора // Успехи физиологических наук. 2021. T. 52, № 1. С. 16–30. EDN: MTXXXZ doi: 10.31857/S0301179821010057

[12] Mazhirina KG, Pervushina ON, Fedorov AA, et al. Neuroimaging and the phenomenon of moral choice. Uspekhi fiziologicheskikh nauk. 2021;52(1):16–30. EDN: MTXXXZ doi: 10.31857/S0301179821010057

[13] Kizyukevich LS. Reactive changes in the kidneys during experimental cholestasis. Grodno: Grodno State Medical University; 2005. 239 p. (In Russ.).

[14] Кизюкевич Л.С. Реактивные изменения в почках при экспериментальном холестазе. Гродно: ГрГМУ, 2005. 239 с.

[15] Kizyukevich LS. Reactive changes in the kidneys during experimental cholestasis. Grodno: Grodno State Medical University; 2005. 239 p. (In Russ.).

[16] Kizyukevich LS. Reasons for the development of multiple organ failure in surgical pathology of the biliary tract. Proceedings National Academy Sciences Belarus. Medical Science Series. 2005;(2):118–121. (In Russ.). EDN: HSHAPL

[17] Кизюкевич Л.С. Причины развития полиорганной недостаточности при хирургической патологии желчевыводящих путей // Вести Национальной академии наук Беларуси. Серия медицинских наук. 2005. № 2. С. 118–121. EDN: HSHAPL

[18] Kizyukevich LS. Reasons for the development of multiple organ failure in surgical pathology of the biliary tract. Proceedings National Academy Sciences Belarus. Medical Science Series. 2005;(2):118–121. (In Russ.). EDN: HSHAPL

[19] Emelyanchik SV, Zimatkin SM. To the modeling of subhepatic cholestasis in rats. News biomedical sciences. 2018;17(2):56–61. (In Russ.).

[20] Емельянчик С.В., Зиматкин С.М. К моделированию подпеченочного холестаза у крыс // Новости медико-биологических наук. 2018. Т. 17, № 2. С. 56–61. EDN: GEZDFT

[21] Emelyanchik SV, Zimatkin SM. To the modeling of subhepatic cholestasis in rats. News biomedical sciences. 2018;17(2):56–61. (In Russ.).

[22] Pirs E. Theoretical and applied histochemistry. Moscow: Izdatel’stvo inostrannoi literatury; 1962. 962 p. (In Russ.).

[23] Пирс Э. Гистохимия теоретическая и прикладная. Москва: Издательство иностранной литературы, 1962. 962 с.

[24] Pirs E. Theoretical and applied histochemistry. Moscow: Izdatel’stvo inostrannoi literatury; 1962. 962 p. (In Russ.).

[25] Zimatkin SM, Macyuk YaR, Mozhejko LA, et al. Histological research methods: a textbook for undergraduates of higher education institutions in the specialty “Histology, cytology, cell biology”. Grodno: Grodno State Medical University; 2015. 190 p. (In Russ.).

[26] Зиматкин С.М., Мацюк Я.Р., Можейко Л.А., и др. Гистологические методы исследования: учебное пособие для магистрантов учреждений высшего образования по специальности «Гистология, цитология, клеточная биология». Гродно: ГрГМУ, 2015. 190 с.

[27] Zimatkin SM, Macyuk YaR, Mozhejko LA, et al. Histological research methods: a textbook for undergraduates of higher education institutions in the specialty “Histology, cytology, cell biology”. Grodno: Grodno State Medical University; 2015. 190 p. (In Russ.).

[28] Paxinos G, Watson C. The rat brain in stereotaxic coordinates. London: Academic Press; 2007. 448 p.

[29] Paxinos G., Watson C. The rat brain in stereotaxic coordinates. London: Academic Press, 2007. 448 p.

[30] Paxinos G, Watson C. The rat brain in stereotaxic coordinates. London: Academic Press; 2007. 448 p.

[31] VV Banin, VL Bykov, editors. Тerminologia Histologica. International terms in human cytology and histology with an official list of Russian equivalents. Moscow: GEOTAR-Media; 2009. 272 p. (In Russ.).

[32] Тerminologia Histologica. Международные термины по цитологии и гистологии человека с официальным списком русских эквивалентов / под ред. В.В. Банина, В.Л. Быкова. Москва: ГЭОТАР-Медиа, 2009. 272 с.

[33] VV Banin, VL Bykov, editors. Тerminologia Histologica. International terms in human cytology and histology with an official list of Russian equivalents. Moscow: GEOTAR-Media; 2009. 272 p. (In Russ.).

[34] Zimatkin SM, Bon EI. “Dark” brain neurons. Morphology. 2017;(6):81–86. (In Russ.). EDN: YLLQDB

[35] Зиматкин С.М., Бонь Е.И. Темные нейроны мозга // Морфология. 2017. № 6. С. 81–86. EDN: YLLQDB

[36] Zimatkin SM, Bon EI. “Dark” brain neurons. Morphology. 2017;(6):81–86. (In Russ.). EDN: YLLQDB

[37] Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (text with EEA relevance). L 276/33. 2010. Official Journal of the European Union. Available from: https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=celex%3A32010L0063 Accessed: 15.01.2024.

[38] Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (text with EEA relevance) // Official Journal of the European Union. L 276/33. 2010. Режим доступа: https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=celex%3A32010L0063 Дата обращения: 15.01.2024.

[39] Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (text with EEA relevance). L 276/33. 2010. Official Journal of the European Union. Available from: https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=celex%3A32010L0063 Accessed: 15.01.2024.

[40] Omelchenko VP, Demidova AA. Computer science, medical informatics, statistics. Moscow: GEOTAR-Media; 2021. 608 p. (In Russ.).

[41] Омельченко В.П., Демидова А.А. Информатика, медицинская информатика, статистика. Москва: ГЭОТАР-Медиа, 2021. 608 с.

[42] Omelchenko VP, Demidova AA. Computer science, medical informatics, statistics. Moscow: GEOTAR-Media; 2021. 608 p. (In Russ.).

[43] Ahmadpour Sh, Behrad A, Fernández-Vega I. Dark neurons: A protective mechanism or a mode of death. J Medical Histology. 2019;3(2):125–131. doi: 10.21608/jmh.2020.40221.1081

[44] Ahmadpour S., Behrad A., Fernández-Vega I. Dark neurons: A protective mechanism or a mode of death // J Medical Histology. 2019. Vol. 3, N 2. Р. 125–131. doi: 10.21608/jmh.2020.40221.1081

[45] Ahmadpour Sh, Behrad A, Fernández-Vega I. Dark neurons: A protective mechanism or a mode of death. J Medical Histology. 2019;3(2):125–131. doi: 10.21608/jmh.2020.40221.1081

[46] Kalimullina LB. On the issue of “dark” and “light” cells. Mophology. 2002;122(4):75–80. (In Russ.).

[47] Калимуллина Л.Б. К вопросу о «темных» и «светлых» клетках // Морфология. 2002. Т. 122, № 4. С. 75–80.

[48] Kalimullina LB. On the issue of “dark” and “light” cells. Mophology. 2002;122(4):75–80. (In Russ.).

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