IMMUNOCYTOCHEMICAL DEMONSTRATION OF TISSUE ANTIGENS AFTER A LONG-TERM STORAGE OF SAMPLES IN METHYLSALICYLATE
D E KORZHEVSKIY , A V HILIAROV
Morphology ›› 2008, Vol. 134 ›› Issue (6) : 76 -79.
IMMUNOCYTOCHEMICAL DEMONSTRATION OF TISSUE ANTIGENS AFTER A LONG-TERM STORAGE OF SAMPLES IN METHYLSALICYLATE
The aim of the present study was to test the possibility of different antigens detection in the biological objects after their storage in methylsalicylate for two or more years before embedding in paraffin, using routine immunocytochemical methods. Using immunocytochemical reactions demostrating intermediate filaments nestin, vimentin, and GFAP, as well as neuronal markers NeuN, neuron-specific enolase, and doublecortin, as the examples, it was shown that long-term storage in methylsalicy-late does not prevent immunocytochemical detection of these antigens.
methylsalicylate / paraffin embedding / immunocy-tochemistry
Eco-Vector
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