A novel population of calretinin-positive, presumably non-neuronal, cells with the polymorphous spines in mouse forebrain

Abstract

Using the immunocytochemical method, the localization of calretinin-positive cells was studied in the frontal sections of anterior portion of the mouse cerebral hemispheres. The population of cells with a characteristic structure, that was not described previously, was detected in the area of anterior horns of lateral brain ventricles. These cells have small (8-10 μm) rounded perikarya which gave rise to 1 or, rarely, 2 nodose processes covered with widely spaced, polymorphous spines (PS) and irregular thickenings. Primary, relatively thick, processes divide to produce thinner processes that also formed thickenings and spines of different dimensions and structure. Calretinin-positive cells with PS (CR+PS) cells are located in the white subcortical matter, layer VI, more rarely in layer V of the frontal area of dorsome-dial cortex close to the cingulum. CR+PS cells were also present in rostro-dorsal part of the caudate nucleus-putamen complex, anterior olfactory nucleus, subependymal layer of the dorso-lateral angle of the lateral ventricle, and, less frequently, near its dorsal wall. In contrast to mouse brain, CR+PS cells were not found in the brain of other animals (rats, rabbits, cats).Within CR+PS cells, no co-localization of calretinin with GABA and other neuronal or glial markers was found. It is suggested that the cells described represent previously unknown, presumably non-neuronal type of the mouse forebrain.

Keywords

calretinin-positive cells / polymorphous spines / non-neuronal brain cells / forebrain / mouse

References

[1] Ревищин А.В., Родионова Е.И. и Леонтович Т.А. Соотношение стриосом и структурных модулей в стриатуме кошки и человека. Докл. РАН, 2000, т. 372, вып. 1, с. 124-126.

[2] Benavides-Piccione R. and DeFelipe J. Different populations of tyrosine-hydroxylase-immunoreactive neurons defined by differential expression of nitric oxide synthase in the human temporal cortex. Cereb. Cortex, 2003, v. 13, p. 297-307.

[3] Cauli B., Audinat E., Lambolez B. et. al. Molecular and physiological diversity of cortical nonpyramidal cells. J. Neurosci., 1997, v. 17, p. 3894-3906.

[4] DeFelipe J. Types of neurons, synaptic connections and chemical characteristics of cells immunoreactive for calbindin-D28K, parvalbumin and calretinin in the neocortex. J. Chem. Neuroanat., 1997, v. 14, p. 1-19.

[5] Gonchar Y. and Burkhalter A. Three distinct families of GABAergic neurons in rat visual cortex. Cereb. Cortex, 1997, v. 7, p. 347-358.

[6] Gonchar Y., Wang Q. and Burkhalter A. Multiple distinct subtypes of GABAergic neurons in mouse visual cortex identified by triple immunostaining. Front. Neuroanat., 2007, v. 1, № 3, p. 1-11.

[7] Jankovski A., Garcia C., Soriano E. et al. Proliferation, migration and differentiation of neuronal progenitor cells in the adult mouse subventricular zone surgically separated from its olfactory bulb. Eur. J. Neurosci., 1998, v. 10, № 12, p. 3853-3868.

[8] Okhotin V.E. and Kalinichenko S.G. Neurons of layer I and their significance in the embryogenesis of the neocortex. Neurosci. Behav. Physiol., 2004, v. 34, № 1, p. 49-66.

[9] Revishchin A.V., Korochkin L.I., Okhotin V.E. et. al. Neural stem cells in the mammalian brain. Int. Rev. Cytol., 2008, v. 265, p. 55-109.

RIGHTS & PERMISSIONS

Eco-Vector