IMMUNOHISTOCHEMICAL CHARACTERISTICS OF THE HYPOPHYSIS UNDER NORMAL AND STRESS-ASSOCIATED CONDITIONS

Abstract

Quantitative immunohistochemical investigation was undertaken to detect the peculiarities of the activation of the hypothalamic-hypophyseal-adrenocortical system at the level of its central unit - the adenohypophysis - in the growing organism under the conditions of chronic exposure to psycho-emotional stressors of varying intensities. Sprague-Dawley rats aged 30 days were exposed to either «mild» or «severe» chronic restraint stress for 5 hours during seven consecutive days. After the last exposure to stress, rats were decapitated, the endocrine glands (pituitary and adrenal glands) were removed, weighted, and embedded in paraffin; histological sections were stained with hematoxylin and eosin. Sections of hypophysis were also stained immunohistochemically using the monoclonal antibodies against adrenocorticotropic hormone (ACTH) and proliferating cell nuclear antigen (PCNA) with subsequent image analysis. The results of the study suggest that the stress-related hyperplasia of corticotropocytes in the hypophyseal pars distalis of the peripuberal rats was mainly associated with differentiation of the immature precursor cells of the hypophysis rather than with the increased cell proliferation

Keywords

hypophysis / adrenal glands / immunohistochemistry / chronic restraint stress

References

[1] Акмаев И.Г., Волкова О.В., Гриневич В.В. и Ресненко А.Б. Эволюционные аспекты стрессорной реакции. Вестн. Росс. акад. мед. наук, 2002, № 6, с. 24-27.

[2] Перт В.К. Суточные и сезонные изменения пролиферативной активности в органах гипофиз-адреналовой системы в норме и при формалиновом стрессе: Автореф. дис. … канд. биол. наук. Тарту, 1990.

[3] Пурсанов А.А. Морфофункциональные эквиваленты реакции аденогипофиза на ограничение двигательной активности. Арх. анат., 1985, т. 84, вып. 10, с. 83-88.

[4] Судаков К.В. Новые акценты классической концепции стресса. Бюл. экспер. биол., 1997, т. 123, № 2, с. 124-130.

[5] Шаляпина В.Г., Бедров Я.А., Ордян Н.Э. и др. Характеристика основных параметров гормональной функции коры надпочечников и ее модификация в онтогенезе у крыс. Журн. эволюц. биохим., 2001, т. 37, № 2, с. 134-138.

[6] Dent G.W., Smith M.A. and Levine S.B. The ontogeny of the neuroendocrine response to endotoxin. Brain Res. Dev. Brain Res., 1999, v. 117, № 1, p. 21-29.

[7] Jasnow A.M., Drazen D.L., Huhman K.L. et al. Acute and chronic social defeat suppresses humoral immunity of male Syrian hamsters (Mesocricetus auratus). Horm. Behav., 2001, v. 40, № 3, p. 428-433.

[8] Kvetnansky R. and Mikulaj L. Adrenal and urinary catecholamines in rats during adaptation to repeated immobilization stress. Endocrinology, 1970, v. 87, № 4, p. 738-743.

[9] Lehmann J., Russig H., Feldon J. and Pryce C.R. Effect of a single maternal separation at different pup ages on the corticosterone stress response in adult and aged rats. Pharmacol. Biochem. Behav., 2002, v. 73, № 1, p. 141-145.

[10] Nolan L.A., Thomas C.K. and Levy A. Enhanced anterior pituitary mitotic response to adrenalectomy after multiple glucocorticoid exposures. Eur. J. Endocrinol., 2003, v. 149, № 2, p. 153-160.

[11] Martí O., Harbuz M.S., Andrés R. et al. Activation of the hypothalamic-pituitary axis in adrenalectomised rats: potentiation by chronic stress. Brain Res., 1999, v. 821, p. 1-7.

[12] Polak J.M. and Van Noorden S. Introduction to immunohistochemistry, 3rd edition, Oxford, Bios Scientific Publishers, 2003.

[13] Sanchez M.M., Aguado F., Sanchez-Toscano F. and Saphier D. Neuroendocrine and immunocytochemical demonstrations of decreased hypothalamo-pituitary-adrenal axis responsiveness to restraint stress after long-term social isolation. Endocrinology, 1998, v. 139, № 2, p. 579-587.

[14] Severino G.S., Fossati I.A., Padoin M.J. et al. Effects of neonatal handling on the behavior and prolactin stress response in male and female rats at various ages and estrous cycle phases of female. Physiol. Behav., 2004, v. 81, № 3, p. 489-498.

[15] Tafet G.E. and Smolovich J. Psychoneuroendocrinological studies on chronic stress and depression. Ann. N. Y. Acad. Sci., 2004, № 1032, p. 276-278.

[16] Tan Z. and Nagata S. PVN c-fos expression, HPA axis response and immune cell distribution during restraint stress. J. Y. Univ. Ocoup. Environ. Health, 2002, v. 24, № 2, p. 131-149.

[17] Van Voorhees E. and Scarpa A. The effects of child maltreatment on the hypothalamic-pituitary-adrenal axis. Trauma Violence Abuse, 2004, v. 5, № 4, p. 333-352.

[18] Viau V. and Sawchenko P.E. Hypophysiotropic neurons of the paraventricular nucleus respond in spatially, temporally, and phenotypically differentiated manners to acute vs. repeated restraint stress. J. Comp. Neurol., 2002, v. 445, № 4, p. 293- 307.

[19] Viveros-Paredes J.M., Puebla-Perez A.M., Gutierrez-Coronado O. et al. Dysregulation of the Th1/Th2 cytokine profile is associated with immunosuppression induced by hypo thalamicpituitary-adrenal axis activation in mice. Int. Immunopharmacol., 2006, v. 6, № 5, p. 774-781.

[20] Wang J., Charboneau R., Barke R.A. et al. Muopioid receptor mediates chronic restraint stress-induced lymphocyte apopto-sis. J. Immunol., 2002, v. 169, № 7, p. 3630-3636.

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