MORPHO-FUNCTIONAL REACTION OF SPLEEN NATURAL KILLER CELLS AND MACROPHAGES TO MELATONIN ADMINISTRATION TO THE ANIMALS KEPT ON DIFFERENT ILLUMINATION REGIMENS
O A Shatskikh , E M Luzikova , O A Shatskykh , Ye M Luzikova
Morphology ›› 2012, Vol. 141 ›› Issue (1) : 43 -46.
MORPHO-FUNCTIONAL REACTION OF SPLEEN NATURAL KILLER CELLS AND MACROPHAGES TO MELATONIN ADMINISTRATION TO THE ANIMALS KEPT ON DIFFERENT ILLUMINATION REGIMENS
The aim this investigation was to study the changes in the numbers of spleen CD57+ and CD68+ cells (natural killer cells and macrophages respectively) after melatonin administration to the animals kept on different illumination regimens. The experimental animals were given melatonin in dose of 0.03 mg per day for 2 and 4 weeks under conditions of natural illumination or artificial darkening. Spleen paraffin sections were stained using immunohistochemical methods for detection of CD57+ and CD68+ cells. It was shown that long-term administration of melatonin under conditions of natural illumination had an immunosuppressive effect, that was manifested by the depopulation of the marginal zones, white pulp and all the zones of the red pulp, parenchyma loosening and denudation of the reticular stroma of the organ. However, long-term hormone administration under conditions of artificial darkening had an immunostimulatory effect as evidenced by the increased inflow of immunocompetent cells into the spleen, their migration from the white pulp into the marginal zones and emigration into peripheral blood flow, concomitant with the increase in the number of lymphoid nodules. The number of CD57+ and CD68+ cells was increased in splenic periarterial lymphoid sheaths and decreased in B-dependent zones of the organ.
spleen / CD57+ cells / CD68+ cells / circadian rhythms / melatonin
| [1] |
Анисимов В.Н. Мелатонин и его место в современной медицине. Русский мед. журн., 2006, т. 14, № 4, с. 269-274. |
| [2] |
Губский В.И. и Губский Л.В. Циркадные ритмы и механизмы циркадной организации. Успехи соврем. биол., 1968, т. 68, с. 23-44. |
| [3] |
Деряпа Н.Р., Мошкин М.П. и Посный В.С. Проблемы медицинской биоритмологии. М., Медицина, 1985. |
| [4] |
Дранник Г.Н. Клиническая иммунология и аллергология. Одесса, АстроПринт, 1999. |
| [5] |
Мендель В.Э. и Мендель О.И. Мелатонин: роль в организме и терапевтические возможности. Опыт применения препарата Мелаксен в российской медицинской практике. Человек и лекарство. Ч. 2, 2010, т. 18, № 6, с. 336-342. |
| [6] |
Парахонский А.П. Влияние мелатонина на иммунную систему. Современные наукоемкие технологии, 2007, № 11, с. 59. |
| [7] |
Bartsch C., Bartsch H., Blask D.E. et al. The Pineal Gland and Cancer. Neuroimmunoendocrine Mechanisms in Malignancy. Berlin: Springer, 2001. |
| [8] |
Currier N.L. and Miller S.C. Echinacea purpurea and melatonin augment natural-killer cells in leukemic mice and prolong life span. J. Altern. Complement. Med., 2001, v. 7, № 3, p. 241-251. |
| [9] |
Fernandez-Tresguerres Hernandez J.A. Effects of hormones on aging of the CNS, the immune system and the skin in rats. An. R. Acad. Nac. Med. (Madr)., 2004, v. 121, № 1, p. 157-176. |
| [10] |
Franca E.L., Feliciano N.D., Silva K.A. et al. Modulatory role of melatonin on superoxide release by spleen macrophages isolated from alloxan-induced diabetic rats. Bratisl. Lek. Listy, 2009, v. 110, № 9, p. 517-522. |
| [11] |
Kim Y.O., Pyo M.Y. and Kim J.H. Influence of melatonin on immunotoxicity of lead. Int. J. Immunopharmacol., 2000, v. 22, № 10, p. 821-832. |
| [12] |
Tian Y.M., Zhang G.Y. and Dai Y.R. Melatonin rejuvenates degenerated thymus and redresses peripheral immune functions in aged mice. Immunol. Lett., 2003, v. 88, № 2, p. 101-104. |
| [13] |
Yellon S.M. and Tran L.T. Photoperiod, reproduction, and immunity in select strains of inbred mice. J. Biol. Rhythms, 2002, v. 17, № 1, p. 65-75. |
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