APPLICATION OF DIFFERENT TYROSINE HYDROXYLASE ANTIBODIES FOR THE STUDY OF CATECHOLAMINERGIC SYSTEMS OF MAMMALIAN BRAIN

I P Grigor'ev; M S Vasilenko; E G Sukhorukova; D E Korzhevskiy; I P Grigoriev; M S Vasilenko; Ye G Sukhorukova; D E Korzhevskiy

doi:10.17816/morph.399398

Morphology ›› 2010, Vol. 138 ›› Issue (6) : 60 -63. DOI: 10.17816/morph.399398

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APPLICATION OF DIFFERENT TYROSINE HYDROXYLASE ANTIBODIES FOR THE STUDY OF CATECHOLAMINERGIC SYSTEMS OF MAMMALIAN BRAIN

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Abstract

Immunohistochemical reaction demonstrating the key enzyme of catecholamine (CA) synthesis - tyrosine hydroxylase (TH) - is currently used as the major morphologic technique for detection of the catecholamine-synthesizing neurons in the brain. At the moment, several monoclonal TH-antibodies are available, which are prepared using different immunogens. The aim of the present study was to compare the results of TH detection in the neurons of rat and human brain using two different antibody clones: TOH A1 and 1В5. Clone TOH A1 antibodies were found to be appropriate for the detection of neurons of the catecholaminergic (CAE) neural centers of the rat. Clone 1В5 antibodies may be used for the visualization of rat and human CA-synthesizing neurons as well as for efferent nerve fibers demonstration in the target areas of CAE innervation.

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brain / antibodies / catecholamines / tyrosine hydroxylase

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I P Grigor'ev, M S Vasilenko, E G Sukhorukova, D E Korzhevskiy, I P Grigoriev, M S Vasilenko, Ye G Sukhorukova, D E Korzhevskiy. APPLICATION OF DIFFERENT TYROSINE HYDROXYLASE ANTIBODIES FOR THE STUDY OF CATECHOLAMINERGIC SYSTEMS OF MAMMALIAN BRAIN. Morphology, 2010, 138(6): 60-63 DOI:10.17816/morph.399398

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