MORPHO-FUNCTIONAL CHARACTERISTIC OF RAT FIBROBLASTS AFTER CULTURE AND ALLOTRANSPLANTATION INTO THE DERMIS
O S Ozerskaya , L I Khozhay , A O Danilov , O S Ozerskaya , L I Khozhai , A O Danilov
Morphology ›› 2010, Vol. 138 ›› Issue (5) : 52 -54.
MORPHO-FUNCTIONAL CHARACTERISTIC OF RAT FIBROBLASTS AFTER CULTURE AND ALLOTRANSPLANTATION INTO THE DERMIS
In model experiments performed on Wistar rats, morphofunctional characteristics of fibroblasts (obtained from the skin of rat pups 2-4 days after their birth) were studied and their viability was estimated shortly after their allotransplantation into the recipient dermis. The results of the research have shown that the suspension of cells received for transplantation after a long-term culture in synthetic media was represented, mainly, by a population of mature fibroblasts. 3 days after transplantation fibroblasts remained viable and preserved their morphological characteristics. This external similarity to the fibroblasts in suspension was associated with the capacity of these cells to proliferate, as indicated by positive immunocytochemical reaction demonstrating proliferating cell nuclear antigen. Later (after 5 days) some part of the grafted fibroblasts also retained their viability and demonstrated heteromorphism, which was caused, probably, by their gradual differentiation into the fibrocytes. In the central portion of the transplant, the destruction of a part of the grafted cells was noted, that was caused by the disturbance of their trophism in the zone of cell crowding.
fibroblasts / dermis / allotransplantation / viability
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