NADPH-DIAPHORASE-POSITIVE STRUCTURES OF THE SPINAL CORD AND SPINAL GANGLIA

V V Porseva; V V Shilkin; V V Porseva; V V Shilkin

doi:10.17816/morph.399321

Morphology ›› 2010, Vol. 137 ›› Issue (2) : 13 -17. DOI: 10.17816/morph.399321

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NADPH-DIAPHORASE-POSITIVE STRUCTURES OF THE SPINAL CORD AND SPINAL GANGLIA

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Abstract

Using the histochemical reaction with the various incubation medium рН, the localization of NADPH-diaphorase was studied in the spinal cord and spinal ganglia of the adult rat. The data obtained indicate, that the application of the incubation medium with рН 7.4 allows to demonstrate the neurons with various NADPH-diaphorase activity, to define not only the density of enzyme distribution, but also to calculate the numbers of NADPH-diaphorase-positive neurons. It was established, that both in the spinal cord and spinal ganglia, NADPH-diaphorase activity was found not in all the neurons: high activity was found in small and medium neurons of the spinal ganglia, while low activity was detected in motoneurons. Other cellular structures of the spinal cord also possessed NADPH-diaphorase activity and were found to be the interneurons.

Keywords

spinal cord / spinal ganglion / NADPH-diaphorase / rat

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V V Porseva,V V Shilkin,V V Porseva,V V Shilkin. NADPH-DIAPHORASE-POSITIVE STRUCTURES OF THE SPINAL CORD AND SPINAL GANGLIA. Morphology, 2010, 137(2): 13-17 DOI:10.17816/morph.399321