CALCIUM- BINDING IBA-1/AIF-1 PROTEIN IN RAT BRAIN CELLS

Abstract

At present, calcium- binding Iba-1 protein is considered to be identical to AIF-1 protein and it is used as one of the selective microglia markers. However, the data on the cell populations capable of expressing Iba-1/AIF-1, are inconsistent. The aims of the present study were to identify rat brain cells expressing Iba-1 and to define their structural characteristics. The methods of immunocytochemistry and confocal laser microscopy were used. Iba-1 protein was detected only in microglial cells, macrophages of brain meninges, supraependymal macrophages, superficial and stromal cells of the choroid plexus -all the cells possessing phagocytotic function. Comparison of the data obtained with the results of other studies allows to suggest that not all of commonly used antibodies against Iba-1/AIF-1 demonstrate the same product. It can not be excluded, that it can be associated with the presence of alternative splicing of Iba-1 mRNA.

Keywords

microglia / Iba-1/AIF-1 protein / immunocytochemistry / brain / choroid plexus

References

[1] Артюхина Н.И. и Саркисова К.Ю. Межполушарная асимметрия повреждений гиппокампа после двусторонней перевязки общих сонных артерий. Рос. физиол. журн., 2004, т. 90, № 2, с. 146-156.

[2] Белецкий В.К. Невроглия, норма и патология. В кн.: Многотомное руководство по патологической анатомии. М., Гос. изд-во мед. лит-ры, 1963, т. II, с. 55-82.

[3] Коржевский Д.Э. Тканевая организация и развитие сосудистого сплетения головного мозга человека. Морфология, 1998, т. 113, вып. 2, с. 105-114.

[4] Коржевский Д.Э. Макрофаги сосудистого сплетения конечного моз га эмбриона человека. Морфология, 2001, т. 119, вып. 1, с. 20-23.

[5] Коржевский Д.Э., Григорьев И.П. и Отеллин В.А. Применение обезвоживающих фиксаторов, содержащих соли цинка, в нейрогистологических исследованиях. Морфология, 2006, т. 129, вып. 1, с. 85-87.

[6] Broglio L., Erne B., Tolnay M. et al. Allograft inflammatory factor- 1: a pathogenetic factor for vasculitic neuropathy. Muscle Nerve., 2008, v.38, № 4, p. 1272-1279.

[7] Deininger M.H., Meyermann R. and Schluesener H.J. The allograft inflammatory factor-1 family of proteins. FEBS Lett., 2002, v. 514, № 2-3, p. 115-121.

[8] Imai Y., Ibata I., Ito D. et al. A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage. Biochem. Biophys. Res. Commun., 1996, v. 224, № 3, p. 855-862.

[9] Ito D., Imai Y., Ohsawa K., et al. Microglia-specific localization of a novel calcium binding protein, Iba1. Brain Res. Mol. Brain Res., 1998, v. 57, № 1, p. 1-9.

[10] Kohler C. Allograft inflammatory factor-1/Ionized calciumbinding adapter moleculer 1 is specifically expressed by most subpopulatios of macrophages and spermatids in testis. Cell Tissue Res., 2007, v. 33, № 2, p. 291-302.

[11] Liu S., Tan W.Y., Chen Q.R. et al. Daintain/AIF-1 promotes breast cancer proliferation via activation of the NF-kappa B/cyclin D1 pathway and facilitates tumor growth. Cancer Sci., 2008, v.99, № 5, p. 952-957.

[12] Mentschel J., Deininger M.H., Schluesener H.J. and Claus R. Effects of malnutrition on the expression of daintain/AIF-1 in the gut mucosa of pigs. J. Vet. Med. Physiol. Clin. Med., 2002, v. 49, № 4, p. 184-188.

[13] Sommerville L.J., Xing C., Kelemen S.E. et al. Inhibition of allograft inflammatory factor-1 expression reduces development of neointimal hyperplasia and p38 kinase activity. Cardiovasc. Res., 2009, v. 81, № 1, p. 206-215.

[14] Wu D., Miyamoto O., Shibuya S. et al. Different expression of macrophages and microglia in rat spinal cord contusion injury model at morphological and regional levels. Acta Med. Okayama., 2005, v. 59, № 4, p. 121-127.

[15] Yamada M., Ohsawa K., Imai Y. et al. X-ray structures of the microglia/macrophage-specific protein Iba-1 from human and mouse demonstrate novel molecular conformation change induced by calcium binding. J. Mol. Biol., 2006, v. 364, № 3, p. 449-457.

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