CYTOCHEMICAL AND ULTRASTRUCTURAL CHARACTERISTIC OF BHK-21CELLS EXPOSED TO DOPAMINE

V S Shubina; V P Lavrovskaya; E N Bezgina; L L Pavlik; D A Moshkov

doi:10.17816/morph.399189

Morphology ›› 2010, Vol. 137 ›› Issue (1) : 5 -9. DOI: 10.17816/morph.399189

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CYTOCHEMICAL AND ULTRASTRUCTURAL CHARACTERISTIC OF BHK-21CELLS EXPOSED TO DOPAMINE

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Abstract

BHK-21 cells were incubated in a medium containing dopamine(DA) and then their catecholamine content evaluated byusing the Falck cytochemical method. The significant intensificationof cell fluorescence as compared to that one in controlpreparations was detected; this effect was proportional to DAconcentration and exposure duration and was more pronouncedin cells in suspension than in those attached to the substrate.Simultaneous ultrastructural investigation has shown that anincreased intensity of the cytoplasm fluorescence correlated withthe appearance of the dense network of fibrils that were morphologicallyidentified as F-actin microfilaments. Prior blockadeof dopaminergic receptors by haloperidol did not change thefollowing DA effect both on the fluorescence intensity and cellultrastructure. The data obtained suggest that DA chronically actingon the living cells was able to penetrate into the cytoplasm,causing actin polymerization and incorporating into the newlyformed actin cytoskeleton. Structurally, this may be manifestedby cytoskeleton and its derivative hypertrophy, that could have asubstantial effect on general morphology of the cell.

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BHK-21 cells dopamine / cells BHK-21 / penetration inside the cytoplasm / cytochemical reaction of Falck

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V S Shubina, V P Lavrovskaya, E N Bezgina, L L Pavlik, D A Moshkov. CYTOCHEMICAL AND ULTRASTRUCTURAL CHARACTERISTIC OF BHK-21CELLS EXPOSED TO DOPAMINE. Morphology, 2010, 137(1): 5-9 DOI:10.17816/morph.399189

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