METHOD FOR QUANTITATIVE EVALUATION OF THE INTENSITY OF THE HISTOCHEMICAL REACTION FOR SULFATED GLYCOSAMINOGLYCANS IN THE TISSUES OF RAT URINARY BLADDER

Abstract

In this paper a method is proposed for the quantitative evaluation of the concentration of sulfated glycosaminoglycans in the tissues of rat bladder using the calibration standards of alcian blue (AB) in 20% aqueous solution of gelatin. The blocks that have the consistency of a dense gel and serve as calibration standards of different dye concentrations, sections 7 and 9 μm thick are cut, and photographed. Using the «Videotest 5.0» program, in the slices with different concentrations of the dye, relative optical density of AB was determined and the graph of its dependence on dye concentration was plotted. On the basis of a calibration graph, it was possible to determine AB concentration in the histological sections of the urinary bladder stained with AB. In the sections of the urinary bladder of the intact rats the concentration of AB in the mucus covering the epithelial layer, was 2.2±1.3 mg/cm3, while in the tissues of its lamina propria it was equal to 2.5±1.0 mg/cm3.

Keywords

histochemistry / alcian blue / urinary bladder / rats

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