CHANGES IN THE FOLLICULAR APPARATUS AND BLOOD VESSELS OF THE OVARIES IN RATS INDUCED BY MELATONIN UNDER CONDITIONS OF HYPERTHERMIA

A. L. Bochkaryova; S. V. Michurina; V. I. Konenkov; I. G. Bochkaryov

doi:10.17816/morph.398938

Morphology ›› 2015, Vol. 148 ›› Issue (5) : 71 -76. DOI: 10.17816/morph.398938

Articles

research-article

CHANGES IN THE FOLLICULAR APPARATUS AND BLOOD VESSELS OF THE OVARIES IN RATS INDUCED BY MELATONIN UNDER CONDITIONS OF HYPERTHERMIA

Author information +

History +

PDF

Abstract

The study was conducted on 155 female Wistar rats (aged 3 months, body mass - 180-200 g), in diestrus phase of the sexual cycle. The effect of the pineal hormone melatonin (MT) on morphological organization of the follicular apparatus and the vasculature of the ovaries was studied in rats after overheating achieving a rectal temperature of 43.5 ºC. It was found that hyperthermic exposure lead to an increase of the relative areas of the arteries, veins and lymph vessels of the ovaries. Overheating caused disturbances of folliculogenesis. The injection of MT after exposure to hyperthermia resulted in a quick (already on Day 7 after exposure) recovery of the disturbances of the venous circulation and lymphatic drainage of the ovary, as evidenced by the normalization of the relative sizes of arteries, veins and lymphatic vessels of the ovary, reaching control values. This, in turn, contributed to the reduction of morphological disorders of folliculogenesis and created the conditions for accelerated recovery of the relative area of generative elements of the ovary (at the stages of primary, preovulatory and atretic follicles) as early as 7 days after hyperthermia.

Keywords

ovaries / follicles / blood and lymph vessels / hyperthermia / melatonin

Cite this article

Download citation ▾

A. L. Bochkaryova, S. V. Michurina, V. I. Konenkov, I. G. Bochkaryov. CHANGES IN THE FOLLICULAR APPARATUS AND BLOOD VESSELS OF THE OVARIES IN RATS INDUCED BY MELATONIN UNDER CONDITIONS OF HYPERTHERMIA. Morphology, 2015, 148(5): 71-76 DOI:10.17816/morph.398938

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Анисимов В. Н., Виноградова И. А. Старение женской репродуктивной системы и мелатонин. СПб.: Система, 2008.

[2]	Арушанян Э. Б. Гормон эпифиза мелатонин и его лечебные возможности // Русск. мед. журн. 2005. Т. 13, № 26. С. 1755-1760.

[3]	Бородин Ю. И. Внутренняя среда организма и корни лимфатической системы. Проблемы лимфангиологии. Новосибирск: Манускрипт, 2010.

[4]	Кветная Т. В., Князькин И. В., Кветной И. М. Мелатонин-нейроиммуноэндокринный маркер возрастной патологии. СПб.: ДЕАН, 2005.

[5]	Комаров Ф. И., Рапопорт С. И., Малиновский Н. К., Анисимов В. Н. Мелатонин в норме и патологии. М.: ИД Медпрактика, 2004.

[6]	Патент РФ № 2165105. Способ экспериментального моделирования общей гипертермии у мелких лабораторных животных / А. В. Ефремов, Ю. В. Пахомова, Е. А. Пахомов, Р. Ш. Ибрагимов, Г. Н. Шорина. Заявка от 22.12.1999 г. Опубл. в БИ. 2001. № 10. С. 308-309.

[7]	Aroyo A., Yavin S., Arav A., Roth Z. Maternal hyperthermia disrupts developmental competence of follicle-enclosed oocytes: in vivo and ex vivo studies in mice // Theriogenology. 2007. Vol. 67, № 5. P. 1013-1021.

[8]	Bagewadikar R. S., Patil M. S. Effect of mild whole body hyperthermia on cytotoxic action of N-metyl-N-nitro-Nnitrosoguanidine in Swiss mice // Toxicol. Lett. 2001. Vol. 121, № 1. P. 63-68.

[9]	Bridges P.J., Brusie M. A., Fortune J. E. Elevated temperature (heat stress) invitro reduces androstenedione and estradiol and increases progesterone secretion by follicular cells from bovine dominant follicles // Domest Anim. Endocrinol. 2005. Vol. 29, № 3. P. 508-522.

[10]	Сruz M. H., Leal C. L., Cruz J. F. et al. Essential actions of melatonin in protecting the ovary from oxidative damage // Theriogenology. 2014. Vol. 82, № 7. P. 925-932.

[11]	Ekmekcioglu C. Melatonin receptors in humans: biological role and clinical relevance // Biomed. Pharmacother. 2006. Vol. 60, № 3. P. 97-108.

[12]	Tanabe M., Tamura H., Taketani T. et al. Melatonin protects the integrity of granulosa cells by reducing oxidative stress in nuclei, mitochondria, and plasma membranes in mice // J. Reprod. Dev. 2015. Vol. 61, № 1. P. 35-41.

[13]	Tseng J. K., Chen C. H., Chou P. C. et al. Influences of follicular size on parthenogenetic activation and in vitro heat shock on the cytoskeleton in cattle oocytes // Reprod. Domest. Anim. 2004. Vol. 39, № 3. P. 146-153.

[14]	Uchida S., Hotta H., Hanada T. et al. Effects of thermal sti mulation, applied to the hindpaw via a hot water bath, upon ovarian blood flow in anesthetized nonpregnant rats // J. Physiol. Sci. 2007. Vol. 57, № 4. P. 227-233.

[15]	Wang S. J., Liu W. J., Wu C. J. et al. Melatonin suppresses apop tosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2) // Theriogenology. 2012. Vol. 78, № 7. P. 1517-1526.