MORPHOLOGICAL CHANGES AFTER THE USE OF MULTIPOTENT STROMAL CELLS OF BONE MARROW ORIGIN TO RESTORE THE LYMPH FLOW IN THE REGION OF THROMBOSED VEIN

Abstract

In the male Wag rats aged 6 months with the body mass of 180-200 g the luminescent microscopy was used to examine the possibility of lymphatic vessel formation after injection into thrombosed vein of the thigh of autologous multipotent stromal cells of bone marrow origin (AMSCBMO) transfected with green fluorescent protein gene. Animals were sacrificed 4 days and 1, 2, 3, 4 and 5 weeks after the injection of AMSCBMO. The control group consisted of intact rats, animals with venous thrombosis receiving no injection of AMSCBMO and those injected with AMSCBMO but without the prior modelling of venous thrombosis. In each experimental and control groups at each time point 11-12 animals were used (total number equal to 226). After the main vein ligation with the subsequent injection of thrombin solution, in addition to the thrombosis of the blood vessels, morphological signs of thrombosis of the lymphatic bed and lymphostasis were present: the dilation of lymphatic vessel lumen, thinning of their layers, intense staining of their luminal heterogeneous content. AMSCBMO, injected into thrombosed vein, were found to directly participate in lymphangiogenesis in the connective tissue around vein, its tissue region and in the area of regional lymph nodes. This is indicated by bright specific luminescence of both individual cells in the wall of the lymphatic vessels, and all their tunics together with the valves, when exposed to UV light.

Keywords

multipotent stromal cells / lymph flow blockade / lymphangiogenesis / venous thrombosis

References

[1] Майбородин И. В., Гаврилин В. Н., Бородин Ю. И., Рейхерт В. Э. Клапаны краевого синуса лимфатических узлов // Морфология. 1996. Т. 110, вып. 6. С. 86-88.

[2] Майбородин И. В., Морозов В. В., Новикова Я. В. и др. Морфологические результаты введения стромальных стволовых клеток костномозгового происхождения в тромбированную вену в эксперименте // Морфология. 2012. Т. 142, вып. 4. С. 54-61.

[3] Майбородин И. В., Якимова Н. В., Матвеева В. А. и др. Морфологический анализ результатов введения аутологичных стволовых стромальных клеток костномозгового происхождения в рубец матки крыс // Морфология. 2010. Т. 138, вып. 6. С. 47-55.

[4] Шевченко Ю. Л., Стойко Ю. М., Лыткина М. И. Основы клинической флебологии. М.: Медицина, 2005.

[5] Arpornmaeklong P., Brown S. E., Wang Z., Krebsbach P. H. Phenotypic characterization, osteoblastic differentiation, and bone regeneration capacity of human embryonic stem cell-derived mesenchymal stem cells // Stem. Cells Dev. 2009. Vol. 18, № 7. P. 955-968.

[6] Cárdenas A., Bataller R., Arroyo V. Mechanisms of ascites formation // Clin. Liver Dis. 2000. Vol. 4, № 2. P. 447-465.

[7] Casley-Smith J. The structure and Functioning of the Blood Vessels, Interstitial Tissues and Lymphatics. Lymphangiology. Stuttgart, New York: Schattauer. 1983. Ch. 2. P. 27-143.

[8] Head J. R., Seeling L. L. Jr. Lymphatic vessels in the uterine endometrium of virgin rats // J. Reprod. Immunol. 1984. Vol. 6, № 3. P. 157-166.

[9] Ikeda R., Michitaka K., Yamauchi Y. et al. Changes in gastrointestinal lymph and blood vessels in patients with cirrhotic portal hypertension // J. Gastroenterol. 2001. Vol. 36, № 10. P. 689-695.

[10] Maiborodin I., Yakimova N., Matveeva V. et al. Angiogenesis in rat uterine scar after introduction after autological mesenchymal stem cells of bone marrow origin // J. Biomed. Sci. Engineering. 2011. Vol. 4, № 3. P. 164-172.

[11] Oikawa H., Masuda T., Sato S. et al. Changes in lymph vessels and portal veins in the portal tract of patients with idiopathic portal hypertension: a morphometric study // Hepatology. 1998. Vol. 27 № 6. P. 1607-1610.

[12] Parasher V. K., Meroni E., Malesci A. et al. Observation of thoracic duct morphology in portal hypertension by endoscopic ultrasound // Gastrointest. Endosc. 1998. Vol. 48, № 6. P. 588- 592.

[13] Stephan S. J., Tholpady S. S., Gross B. et al. Injectable tissue-engineered bone repair of a rat calvarial defect // Laryngoscope. 2010. Vol. 120, № 5. P. 895-901.

[14] Tasso R., Fais F., Reverberi D. et al. The recruitment of two consecutive and different waves of host stem/progenitor cells during the development of tissue-engineered bone in a murine model // Biomaterials. 2010. Vol. 31, № 8. P. 2121-2129.

[15] Wessler S., Reimer S. M., Sheps M. C. Biological assay of a thrombosis inducing activity in human serum // J. Appl. Physiol. 1959. Vol. 14. P. 943-946.

[16] Yamauchi Y., Ikeda R., Michitaka K. et al. Morphometric analysis of lymphatic vessels in primary biliary cirrhosis // Hepatol. Res. 2002. Vol. 24, № 2. P. 107.

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