AGE-RELATED PECULIARITIES OF THE NEURONS CONTAINING DIFFERENT TYPES OF CALCIUM-BINDING PROTEINS IN THE INTRAMURAL GANGLIA OF SMALL INTESTINE

Abstract

Immunohistochemical and morphometric methods were used to study the localization, relative content and the cross-sectional area of calbindin (CB)- and calretinin (CR)-immunopositive neurons in the intramural ganglia of the myenteric plexus of duodenum in rats (n=37) in postnatal ontogenesis (Days 1, 10, 20, 30, 60, 1 and 2 years). CB - and CR-immunopositive neurons were detected in all the rats studied, from newborn to aged ones. The proportion of CR-immunopositive neurons was increased during the first 10 days of life, and then was not changed significantly, including the older animals. The proportion of CB-containing neurons increased, reaching a maximum value by Day 20, then declined insignificantly by Day 30 and was not significantly changing thereafter. The average size of CB- and CR-immunopositive neurons was significantly greater than the average cross-sectional area of immunoreative neurons in all age groups.

Keywords

small intestine / intramural ganglia / calbindin / calretinin / ontogenesis

References

[1] Корзина М. Б., Коробкин А. А., Васильева О. А. и Маслюков П. М. Морфологические особенности звездчатого узла белой крысы. Морфология, 2010, т. 137, вып. 2, с. 23-26.

[2] Маслюков П. М., Коробкин А. А., Коновалов В. В. и др. Возрастное развитие кальбиндин-иммунопозитивных нейронов симпатических узлов крысы, Морфология, 2012, т. 141, вып. 1, с. 77-80.

[3] Маслюков П. М., Ноздрачев А. Д. и Timmermans J. P. Возрастные особенности нейротрансмиттерного состава нейронов звездчатого узла. Росс. физиол. журн. им. И. М. Сеченова, 2006, т. 92, № 2, с. 214-221.

[4] Andressen C., Blumcke I. and Celio M. R. Calcium-binding proteins: selective markers of nerve cells. Cell Tissue Res., 1993, v. 271, p. 181-208.

[5] Bellido T., Huening M., Raval-Pandya M. et al. Calbindin-D28k is expressed in osteoblastic cells and suppresses their apoptosis by inhibiting caspase-3 activity. J. Biol. Chem., 2000, v. 275, p. 26328-26332.

[6] Chard P. S., Bleakman D., Christakos S. et al. Calcium buffering properties of calbindin-D28k and parvalbumin in rat sensory neurons. J. Physiol., 1993, v. 472, p. 341-357.

[7] Endo T. and Onaya T. Immunohistochemical localization of parvalbumin in rat and monkey autonomic ganglia. J. Neurocytol. 1988, v. 17, p. 73-77.

[8] Franconville R., Revet G., Astorga G. et al. Somatic calcium level reports integrated spiking activity of cerebellar interneurons in vitro and in vivo. J. Neurophysiol., 2011, v. 106, p. 1793-1805.

[9] Lee D., Obukhov A. G., Shen Q. et al. Calbindin-D28k decreases L-type calcium channel activity and modulates intracellular calcium homeostasis in response to K+ depolarization in a rat beta cell line RINr1046-38. Cell Calcium, 2006, v. 39, p. 475- 485.

[10] Masliukov P. M. Sympathetic neurons of the cat stellate ganglion in postnatal ontogenesis: morphometric analysis. Auton. Neurosci., 2001, v. 89, № 1-2, p. 48-53.

[11] Masliukov P.M., Korobkin A. A., Nozdrachev A. D. and Timmermans J. P. Calbindin-D28k immunoreactivity in sympathetic ganglionic neurons during development. Auton. Neurosci., 2012, v. 167, № 1-2, p. 27-33.

[12] Mitsui R. Immunohistochemical analysis of substance P-containing neurons in rat small intestine. Cell Tissue Res., 2011, v. 343, p. 331-341.

[13] Sayegh A. I. and Ritter R. C. Morphology and distribution of nitric oxide synthase-, neurokinin-1 receptor-, calretinin-, calbindin-, and neurofilament-M-immunoreactive neurons in the myenteric and submucosal plexuses of the rat small intestine. Anat. Rec. A Discov. Mol. Cell Evol. Biol., 2003, v. 271, № 1, p. 209-216.

[14] Schwaller B. The regulation of a cell’s Ca(2+) signaling toolkit: the Ca (2+) homeostasome. Adv. Exp. Med. Biol., 2012, v. 740, p. 1-25.

[15] Yano S., Tokumitsu H. and Soderling T. R. Calcium promotes cell survival through CaM-K kinase activation of the proteinkinase-B pathway. Nature, 1998, v. 396, p. 584-587.

RIGHTS & PERMISSIONS

Eco-Vector