INTERRELATIONS OF PINEAL GLAND MORPHO-FUNCTIONAL INDICES AND IMMUNE SYSTEM ORGANS IN RATS EXPOSED TO NATURAL ILLUMINATION REGIME AND CONTINUOUS ILLUMINATION

Abstract

The objective of this investigation was to determine the complex response of the pineal gland (PG) and of the organs of the immune system in Wistar rats in response to a violation of the illumination regime in the experiment. Animals were kept under natural light regime and continuous illumination for 14 days. After that rats were sacrificed and the mass of the body, PG, gonads, thymus and spleen was measured. Thymus and spleen cell subpopulations were determined by flow cytometry. The lipofuscin content in PG was determined by measuring an auto-fluorescence intensity in frozen tissue sections in the wavelength range of 505-545 nm using a confocal laser scanning microscope LSM 510 META (Carl Zeiss). The correlation analysis showed an increase in the amount and the change of sign and direction of relations between the indices of the state of PG and the immune system. This indicates the up-regulation of the intensity of intersystem relationships and the change of migration and differentiation vector of immunocompetent cells.

Keywords

lymphocytes / pineal gland / lipofuscin / continuous illumination

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