DECALCIFICATION IN FORMIC ACID AFTER FIXATION IN ZINC-ETHANOLFORMALDEHYDE DOES NOT PRECLUDE IDENTIFICATION OF NEURONAL AND GLIAL MARKERS

E. A. Kolos; D. E. Korzhevskiy

doi:10.17816/morph.398706

Morphology ›› 2013, Vol. 144 ›› Issue (4) : 076 -079. DOI: 10.17816/morph.398706

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DECALCIFICATION IN FORMIC ACID AFTER FIXATION IN ZINC-ETHANOLFORMALDEHYDE DOES NOT PRECLUDE IDENTIFICATION OF NEURONAL AND GLIAL MARKERS

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Abstract

The aim of this study was to determine the possibility of immunohistochemical demonstration of various tissue antigens in paraffin sections of zinc-ethanol-formaldehyde-fixed spinal cord, surrounded by the vertebral tissues and adjacent muscles, after decalcification in formic acid, in adult (n=5) and newborn (n=2) Wistar rats. In the present work, the antibodies used were against: glial fibrillary acidic protein, neurofilaments, neuronal specific nuclear protein, enzyme tyrosine hydroxylase, protein of synaptic vesicles — synaptophysin and the intermediate filament protein — vimentin. Combination of fixation in zinc-ethanolformaldehyde and decalcification in 21% formic acid solution was shown to provide an optimal preservation of the antigens studied.

Keywords

immunohistochemistry / decalcification / fixation in zinc-ethanol-formaldehyde

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E. A. Kolos, D. E. Korzhevskiy. DECALCIFICATION IN FORMIC ACID AFTER FIXATION IN ZINC-ETHANOLFORMALDEHYDE DOES NOT PRECLUDE IDENTIFICATION OF NEURONAL AND GLIAL MARKERS. Morphology, 2013, 144(4): 076-079 DOI:10.17816/morph.398706

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