METHOD OF IMMUNOCYTOCHEMICAL DEMONSTRATION OF CHOLINERGIC NEURONS IN THE CENTRAL NERVOUS SYSTEM OF LABORATORY ANIMALS

D. E. Korzhevskiy; I. P. Grigoriyev; O. V. Kirik; N. M. Zelenkova; Ye. G. Sukhorukova

doi:10.17816/morph.398705

Morphology ›› 2013, Vol. 144 ›› Issue (6) : 069 -072. DOI: 10.17816/morph.398705

Articles

research-article

METHOD OF IMMUNOCYTOCHEMICAL DEMONSTRATION OF CHOLINERGIC NEURONS IN THE CENTRAL NERVOUS SYSTEM OF LABORATORY ANIMALS

Author information +

History +

PDF

Abstract

A protocol of immunocytochemical demonstration of choline acetyltransferase (ChAT), a key enzyme of acetylcholine synthesis, in paraffin sections of the brain of some laboratory animals, is presented. The method is simple, gives fairly reproducible results and allows for demonstration of ChAT in neurons, nerve fibers, and terminals in preparations of at least three species of laboratory animals including rat, rabbit, and cat. Different kinds of fixation (10% formalin, 4% paraformaldehyde, or zinc-ethanol-formaldehyde) were found suitable for immunocytochemical visualization of ChAT, however, optimal results were obtained with the application of zinc-ethanol-formaldehyde

Keywords

central nervous system / cholinergic neurons / choline acetyltransferase / immunocytochemistry

Cite this article

Download citation ▾

D. E. Korzhevskiy, I. P. Grigoriyev, O. V. Kirik, N. M. Zelenkova, Ye. G. Sukhorukova. METHOD OF IMMUNOCYTOCHEMICAL DEMONSTRATION OF CHOLINERGIC NEURONS IN THE CENTRAL NERVOUS SYSTEM OF LABORATORY ANIMALS. Morphology, 2013, 144(6): 069-072 DOI:10.17816/morph.398705

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Коржевский Д. Э. и Юмкина Е. А. Применение методов демаскирования антигенов на парафиновых срезах головного мозга крысы. Морфология, 2005, т. 127, вып. 2, с. 76–77.

[2]	Отеллин В. А. и Григорьев И. П. Синаптические и несинаптические межнейронные связи как структурно-медиаторная основа функций неостриатума. В кн.: Стриарная система и поведение в норме и патологии. Л., изд. Ин-та физиологии им. И. П. Павлова АН СССР, 1984, с. 138–146.

[3]	Daulatzai M. A. Early stages of pathogenesis in memory impairment during normal senescence and Alzheimer`s disease. J. Alzheimer Dis., 2010, v. 20, № 2, p. 355–367.

[4]	Mechawar N., Cozzari C. and Descarries L. Cholinergic innervation in adult rat cerebral cortex: a quantitative immunocytochemical description. J. Comp. Neurol., 2000, v. 428, № 2, p. 305–318.

[5]	Picciotto M. R., Higley M. J. and Mineur Y. S. Acetylcholine as a neuromodulator : cholinergic signaling shapes nervous system function and behavior. Neuron, 2012, v. 76, № 1, p. 116–129.