STRUCTURAL AND HISTOCHEMICAL CHANGES IN THE RAT CEREBELLUM PURKINJE CELLS AFTER CHOLESTASIS

Abstract

The aim of the study was the estimation of structural and metabolic changes in the rat cerebellum Purkinje cells (PC) in the dynamics of subhepatic cholestasis. Histological, histochemical and electron microscopic methods were used. It was found that the cholestasis induced the progressive intensification of the structural and metabolic disturbances in the cerebellar PC reaching their maximum on days 10–20 and resulting in PC death and PC number reduction. In the cytoplasm of these cells the reduction of the activities of succinate dehydrogenase, NADH-diaphorase, glucose-6-dehydrogenase was recorded together with the decrease of RNA content and the activation of lactate dehydrogenase and acid phosphatase. The disturbances of PC at the ultrastructural level included the destruction of the nucleus and organelles (especially, of the mitochondria), associated with the increase of phagosome size and number, augmentation of relative free ribosome number, appearance of close contacts between mitichondria and cell nucleus and organelles. After 45–90 days in survived animals gradual normalization of PC structure and metabolism took place apparently as a result of spontaneous cholestasis elimination due to the development of new bile ducts.

Keywords

cerebellum / Purkinje cells / structure / metabolism / cholestasis

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