THE APPLICATION OF A «PIXEL METHOD» FOR THE QUANTITATIVE ASSESSMENT OF THE RESULTS OF THE HISTOCHEMICAL STUDIES
Viktor Mikhaylovich Chertok , Marina Sergeevna Startseva , Aleksandr Evgen'evich Kotsyuba , V M Chertok , М S Startseva , A Ye Kotsyuba
Morphology ›› 2012, Vol. 142 ›› Issue (5) : 71 -75.
THE APPLICATION OF A «PIXEL METHOD» FOR THE QUANTITATIVE ASSESSMENT OF THE RESULTS OF THE HISTOCHEMICAL STUDIES
The results of the application of a pixel method for the automated measurement of the intensity of histochemical reaction in the neurons are presented. The method is based on measuring principle, which includes the automatic counting the sum of brightnesses of all the pixels forming the image with the help of standard computer programs. The potential of this method is demonstrated on the example of NADPH-diaphorase activity study in the nitroxidergic neurons of sensory and motor nuclei of the medulla of healthy rats.
quantitative histochemistry / the pixel method / nitroxidergic neurons
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