MICROGLIA DETECTION IN THE BRAIN PREPARATIONS AFTER LONG-TERM STORAGE IN FORMALIN
Elena Gennadievna Sukhorukova , Mikhail Sergeevich Zakhryapin , Nikolay Mil'evich Anichkov , Dmitriy Eduardovich Korzhevskiy , Ye G Sukhorukova , M S Zakhryapin , N M Anichkov , D E Korzhevskiy
Morphology ›› 2012, Vol. 142 ›› Issue (5) : 68 -71.
MICROGLIA DETECTION IN THE BRAIN PREPARATIONS AFTER LONG-TERM STORAGE IN FORMALIN
Relatively simple protocol for identification of microgliocytes in archived material is proposed. A novel variant is based on preparation processing with the use of polyclonal antibodies against Iba-1 protein. The proposed approach includes antigen heat unmasking in a modified citrate buffer and prolonged incubation of the sections with the primary antibodies at high temperature. The method is highly specific and allows selective detection of microgliocytes in sections prepared from a material stored in formalin for 2 months.
formalin fixation / microglia / Iba-1 protein
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