METHODICAL APPROACHES TO EXPERIMENTAL STUDY OF HISTOPHYSIOLOGY OF MUCOCILIARY TRANSPORT SYSTEM IN THE UTERINE TUBES

A. V. Pavlov , T. V. Korablyova , L. I. Yesev , O. A. Fokanova , Yu. A. Lukashevich

Morphology ›› 2019, Vol. 155 ›› Issue (1) : 60 -65.

PDF
Morphology ›› 2019, Vol. 155 ›› Issue (1) : 60 -65. DOI: 10.17816/morph.398501
Articles
research-article

METHODICAL APPROACHES TO EXPERIMENTAL STUDY OF HISTOPHYSIOLOGY OF MUCOCILIARY TRANSPORT SYSTEM IN THE UTERINE TUBES

Author information +
History +
PDF

Abstract

Objective - to develop the methods for experimental study of the mucociliary system of the uterine tubes based on the use of modern digital technologies. Materials and methods. Using the complex of histological and specialized “Azimuth” (Russia) hardware-software system, the structural and functional organization of the uterine tubes of small laboratory animals was examined. Results and Conclusions. The methodical approaches are proposed which allow to carry out the complex morpho-functional analysis including the intravital study of the mucus flow velocity at the level of the organ and in the parietal layer, the parameters of motor activity of the ciliary apparatus, the linear sizes of microscopic objects in combination with the histological examination of the uterine tubes.

Keywords

uterine tubes / epithelial layer / ciliary beat frequency / mucus flow velocity / morphometry

Cite this article

Download citation ▾
A. V. Pavlov, T. V. Korablyova, L. I. Yesev, O. A. Fokanova, Yu. A. Lukashevich. METHODICAL APPROACHES TO EXPERIMENTAL STUDY OF HISTOPHYSIOLOGY OF MUCOCILIARY TRANSPORT SYSTEM IN THE UTERINE TUBES. Morphology, 2019, 155(1): 60-65 DOI:10.17816/morph.398501

登录浏览全文

4963

注册一个新账户 忘记密码

References

[1]

Алексеев Д. С., Попечителев Е. П. Методы исследования двигательной активности мукоцилиарного аппарата // Известия ЮФУ. Технические науки. Тематический выпуск. 2012. С. 189-194.

[2]

Кобылянский В. И. Мукоцилиарная система. Фундаментальные и прикладные аспекты. М.: БИНОМ. 2008. 416 с.

[3]

Козлов В. С., Крамной А. И., Аверин А. А., Лукашевич Ю. А., Алексеев Д. С. Исследование двигательной активности цилиарного аппарата мерцательного эпителия полости носа in vitro // Российская ринология. 2005. № 3. С. 30-33.

[4]

Павлов А. В., Есев Л. И. Методические подходы к комплексному изучению функциональной морфологии эпителиальной выстилки трахеи в эксперименте // Морфология, 2012. Т. 142, вып. 6. С.73-76.

[5]

Bylander A., Nutu M., Wellander R., Goksör M., Billig H., Larsson D. G. Rapid effects of progesterone on ciliary beat frequency in the mouse fallopian tube // Reprod. Biol. Endocrinol. 2010. Vol. 8. P. 48. doi: 10.1186/1477-7827-8-48.

[6]

Ezzati M., Djahanbakhch O., Arian S., Carr B. R. Tubal transport of gametes and embryos: a review of physiology and pathophysiology.// J. Assist.Reprod. Genet. 2014. Vol.31, № 10. P. 1337-1347. doi: 10.1007/s10815-014-0309-x.

[7]

Kölle S., Dubielzig S., Reese S., Wehrend A., König P., Kummer W. Ciliary transport, gamete interaction, and effects of the early embryo in the oviduct: ex vivo analyses using a new digital videomicroscopic system in the cow // Biol. Reprod. 2009. Vol. 81, № 2. P. 267-274. doi: 10.1095/biolreprod.108.073874.

[8]

Lyons R.A., Saridogan E., Djahanbakhch O. The reproductive significance of human Fallopian tube cilia // Hum. Reprod. 2006. Vol. 12, № 4. P. 363-372.

[9]

Noreikat K., Wolff M., Kummer W., Kölle S. Ciliary activity in the oviduct of cycling, pregnant, and muscarinic receptor knockout mice // Biol. Reprod. 2012. Vol. 86, № 4. P. 1-10. doi: 10.1095/ biolreprod.111.096339.]

[10]

Raidt J., Werner C., Menchen T., Dougherty G. W., Olbrich H., Loges N. T., Schmitz R., Pennekamp P., Omran H. Ciliary function and motor protein composition of human fallopian tubes // Hum. Reprod. 2015. Vol. 30, № 12. P. 2871-2880. doi: 10.1093/humrep/dev227.

[11]

Shi D., Komatsu K., Uemura T., Fujimori T. Analysis of ciliary beat frequency and ovum transport ability in the mouse oviduct // Genes Cells. 2011. Vol. 16, № 3. P. 282-290. doi: 10.1111/j.13652443.2011.01484.x.

RIGHTS & PERMISSIONS

Eco-Vector

AI Summary AI Mindmap
PDF

119

Accesses

0

Citation

Detail

Sections
Recommended

AI思维导图

/