PERIPHERAL BLOOD NEUTROPHIL MORPHOLOGY IN THE AMERICAN MINK (NEOVISON VISON) OF VARIOUS COLORS: A MORPHOMETRIC STUDY

Abstract

Objective - to examine the effect of aleutian gene (a/a) on the morphology of blood neutrophils in American mink (Neovison vison) of various colors. Materials and methods. The study was performed on American minks (Neovison vison) of several colors (n=92, 6 months-old animals). The morphology of neutrophils was studied in the peripheral blood smears using light microscopy. Abnormal granule size and numbers were determined by morphometric analysis in homozygous minks with aleutian gene (a/a). Results. The neutrophils of leukocytes in minks without the aleutian gene (a/a) in a homozygous state were morphologically similar to those in most other mammalian species. In minks carrying the recessive (a/a) mutation: aleutian (a/a), sapphire (a/a p/p), lavender (m/m a/a), violet (m/m a/a p/p), Shadow sapphire (SH/+a/a p/p), sapphire Leopard (SK/+a/a p/p), Cross sapphire (S/+a/a p/p) - the neutrophils had abnormally large granules. Morphometric analysis revealed the significantly differences in size and numbers of the abnormal neutrophilic granules between minks of various colors. Among mink genotypes studied, the most expressed abnormalities were expressed in violet minks (m/m a/a p/p) due to the greatest number of «giant» granules in their neutrophils that had the smallest size as compared with those in other mink mutant forms. Conclusions. The aleutian gene (a/a) affects the structure of blood neutrophils in all mink colors. The defect Neutrophil granule abnormality is modified by genetic environment, which affects the coat color in minks.

Keywords

peripheral blood neutrophils / American mink (Neovison vison) / aleutian gene (a/a / coat color mutations)

References

[1] Кижина А. Г., Узенбаева Л. Б., Илюха В. А., Тютюнник Н. Н. Активность ферментов лейкоцитов американской норки (Neovison vison): генотипические и возрастные особенности // Труды КарНЦ РАН. 2016. № 11. С. 88-96. Doi: 10.17076/ eb435.

[2] Колдаева Е. М., Колдаев Н. А. Доместикация и хозяйственно полезные признаки у пушных зверей // Информ. вестник ВОГиС. 2007. Т. 11, № 1. С. 62-75.

[3] Трапезов О. В. Регуляторные эффекты генов поведения и управление окрасочным формообразованием у американских норок (Mustela vison Schreber, 1977) // Информ. вестник ВОГиС. 2008. Т. 12, № 1/2. С. 63-83.

[4] Узенбаева Л. Б., Трапезов О. В., Кижина А. Г., Илюха В. А., Трапезова Л. И., Тютюнник Н. Н. Влияние мутаций, затрагивающих окраску меха, на структуру лейкоцитов крови у американской норки (Mustela vison Schreber, 1777) // Генетика. 2011. Т. 47, № 1. С. 87-94.

[5] Этическая экспертиза биомедицинских исследований. Практические рекомендации / Под ред. Ю. Б. Белоусова. М., 2005. 156 с.

[6] Andrews T., Sullivan K. E. Infection in patients with inherited defects in phagocytic function // Clin. Microbiol. Rev. 2003. Vol. 16, № 4. P. 597-621.

[7] Anistoroaei R., Krogh A. K., Christensen K. A. Frameshift mutation in the LYST gene is responsible for the Aleutian color and the associated Chediak-Higashi syndrome in American mink // Animal. Genet. 2013. Vol. 44, № 2. P. 178-183.

[8] Вalint B., Bhatia K. P. Parkinsonism and other movement disorders associated with Chediak-Higashi syndrome: case report and systematic literature review // Mov. Disord. 2015. Vol. 2, № 1. P. 93-98.

[9] Declaration of Helsinki. 29thWorld Medical Association General Assembly, Tokyo, Japan, October, 1975; 52nd World Medical Association General Assembly, Edinburgh, Scotland, October 2000.

[10] Hammer A. S., Andersen T. H., Eriksen T., Kortegaard H. E., Diets H. H., Chriel M. Radiographic evaluation of destructive periodontal disease in blue mink in relation to age and blood morphology // Can. J. Vet. Res. 2005. Vol. 69. P. 128-134.

[11] Holland P., Torgersen M. L., Sandvig K. A., Simonsen A. LYST affects lysosome size and quantity but not trafficking or degradation through autophagy or endocytosis // Traffic. 2014. Vol. 15. P. 1390-1405.

[12] Huizing M., Helip-Wooley A., Westbroek W., Gunay-Aygun M., Gahl W.A. Disorders of lysosome-related organelle biogenesis: clinical and molecular genetics // Annu. Rev. Genomics Hum. Genet. 2008. Vol. 9. P. 359-386.

[13] Karim M. A., Suzuki K., Fukai K., Oh J., Nagle D. L., Moore K. J., Barbosa E., Falik-Borenstein T., Filipovich A., Ishida Y., Kivrikko S., Klein C., Kreuz F., Levin A., Miyajima H., Re gueiro J., Russo C., Uyama E., Vierimaa O., Spritz R. A. Appa rent genotype - phenotype correlation in childhood, adolescent, and adult Chediak-Higashi syndrome // Am. J. Med. Genet. 2002. Vol. 108. P. 16-22.

[14] Spicer S. S., Sato A., Vincent R., Eguchi M, Poon K. C. Lysosome enlargement in the Chediak-Higashi syndrome // Federation Proc. 1981. Vol. 40, № 5. P. 1451-1455.

RIGHTS & PERMISSIONS

Eco-Vector