STRUCTURAL AND BIOMECHANICAL CHARACTERISTICS OF BLOOD NEUTROPHILS IN RATS EXPOSED TO TITANIUM DIOXIDE NANOPARTICLES (ATOMIC FORCE MICROSCOPIC STUDY)

L. A. Sharafutdinova; Yu. S. Zamula; Z. R. Khismatullina; M. R. Daminov; V. V. Valiullin

doi:10.17816/morph.398356

Morphology ›› 2018, Vol. 154 ›› Issue (4) : 40 -45. DOI: 10.17816/morph.398356

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STRUCTURAL AND BIOMECHANICAL CHARACTERISTICS OF BLOOD NEUTROPHILS IN RATS EXPOSED TO TITANIUM DIOXIDE NANOPARTICLES (ATOMIC FORCE MICROSCOPIC STUDY)

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Abstract

Objective - to study the structural and biomechanical characteristics of blood neutrophils after exposure to titanium dioxide (TiO2) nanoparticles. Materials and methods. Blood neutrophils obtained from 20 healthy donors of both sexes aged 20 to 22 years were studied in vitro before and after exposure to TiO2 nanoparticles. Using atomic force microscopy, structural and biomechanical characteristics of neutrophils (diameter, cell height, roughness, stiffness) were examined. Results. The structural changes of neutrophils after exposure to TiO2 nanoparticles were observed which consisted of decline of cell diameter and an increase of neutrophil height, rigidity and degree of roughness of their surface. Conclusion. The changes in the structural and biomechanical properties of neutrophils observed indicate the possible cytotoxic effect of TiO2 nanoparticles.

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neutrophils / atomic force microscopy / nanoparticles / titanium dioxide

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L. A. Sharafutdinova, Yu. S. Zamula, Z. R. Khismatullina, M. R. Daminov, V. V. Valiullin. STRUCTURAL AND BIOMECHANICAL CHARACTERISTICS OF BLOOD NEUTROPHILS IN RATS EXPOSED TO TITANIUM DIOXIDE NANOPARTICLES (ATOMIC FORCE MICROSCOPIC STUDY). Morphology, 2018, 154(4): 40-45 DOI:10.17816/morph.398356

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