DETECTION OF MORPHOLOGICAL SIGNS OF MAST CELL DEGRANULATION IN HUMAN CHOROID PLEXUS USING DIFFERENT STAINING METHODS AND IMMUNOHISTOCHEMISTRY

Abstract

Objective - to determine the signs of degranulation of mast cells of the human choroid plexus using toluidine blue staining, histochemical reaction to glycosaminoglycans and sulfaminoglycans by alcian blue and immunohistochemical reaction to mast cell tryptase. Materials and methods. The study was performed on fragments of the choroid plexus of the brain taken from 29 individuals aged 14-73 years. To assess the number and location of mast cells, some of the slides were stained with toluidine blue and alcian blue with nuclear fast red counterstaining. Also, immunohistochemical reaction to the human mast cell tryptase was carried out. Results. The applied methods of staining showed different degree of effectiveness in detecting mast cells and their degranulation. It was found that the best method for visualization of mast cells (both granulated and degranulating) in the choroid plexus was tryptase immunohistochemistry. About 60 % of the mast cells in the choroid plexus in the specimens studied were degranulating cells surrounded by an immunopositive halo showing a pericellular localization of tryptase. Conclusions. Tryptase immunohistochemistry was found to be most effective for demonstration mast cells in the choroid plexus of the human brain in comparison to classical methods of toluidine blue and alcian blue staining. The data obtained indicate that degranulation of mast cells in the human choroid plexus belongs to a type of piecemeal degranulation that permanently affects the functional activity of the plexus and, accordingly, the blood-CSF barrier.

Keywords

mast cells / mast cell tryptase / degranulation choroid plexus / brain

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