MORPHOMETRIC PARAMETERS OF HEPATOCYTES IN THREE ZONES OF MOUSE HEPATIC LOBULES IN THE RECOVERY PERIOD AFTER SHIN BONES FRACTURE AND AMINO ACID COMPLEX TREATMENT

Abstract

Objective - to detect the dynamics of the morphometric parameters of hepatocytes after the fracture of the shin bones and to assess the effect of the amino acid mixture on the recovery processes in the liver. Material and methods. Liver was studied in 66 male CBA mice at the age of 2-3 months, divided into 3 experimental groups (54 mice with shin bones fracture) and 1 control group (12 intact mice). The volume of the mononuclear hepatocytes and their nuclei, nuclear-cytoplasmic index, the content of binucleated hepatocytes and their mitotic activity were measured. Observations were conducted on Days 3, 7 and 28 after bone fracture. During the recovery period, mice of the experimental groups received either a standard diet, protein-poor diet or a diet supplemented by an amino acid mixture (with equal weight ratios of leucine, isoleucine, arginine, methionine). Results. Fracture of shin bones was accompanied by a change in the morphometric parameters of hepatocytes, mediated by the organ response to trauma. Peripheral and central zones of the hepatic lobe served as a source of liver reparative regeneration. The use of the amino acid mixture had a stimulating effect on the development of recovery processes in the liver. Conclusions. The liver responds to the bone fractures with the changes of morpho-functional indices of hepatocytes, which differ between the individual zones of the lobules. Dynamics of regenerative processes in the liver depends on the nature of the diet.

Keywords

hepatocyte / morphometry / liver / regeneration / bone fracture

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