DEMONSTRATION OF WEIBEL-PALADE BODIES USING VON WILLEBRAND FACTOR IMMUNOCYTOCHEMISTRY AND CONFOCAL LASER MICROSCOPY

Ye. G. Sukhorukova; O. V. Kirik; D. E. Korzhevskiy

doi:10.17816/morph.398199

Morphology ›› 2018, Vol. 153 ›› Issue (1) : 71 -75. DOI: 10.17816/morph.398199

Articles

research-article

DEMONSTRATION OF WEIBEL-PALADE BODIES USING VON WILLEBRAND FACTOR IMMUNOCYTOCHEMISTRY AND CONFOCAL LASER MICROSCOPY

Author information +

History +

PDF

Abstract

Objective - to explore the possibility of application of immunocytochemical reaction to von Willebrand factor to analyze the localization of Weibel-Palade bodies in the venous endothelium using confocal laser microscopy. Material and methods. The study was performed on paraffin sections of human great saphenous vein (n=5). Results. A comparative study of preparations for light and confocal laser microscopy suggests that the confocal laser microscopy due to the possibility to achieve high resolution allows identification of the localization of the product in more detail, as well as to carry out a quantitative analysis. Conclusions. The presented set of reagents for immunofluorescent reaction provides high quality preparations, in which individual Weibel-Palade bodies are readily identifiable Application of the proposed set of fluorochromes for confocal laser microscopy allows easy separation of channels and the study of structures independently of each other.

Keywords

endothelium / Weibel-Palade bodies / von Willebrand factor / immunocytochemistry / confocal laser microscopy

Cite this article

Download citation ▾

Ye. G. Sukhorukova, O. V. Kirik, D. E. Korzhevskiy. DEMONSTRATION OF WEIBEL-PALADE BODIES USING VON WILLEBRAND FACTOR IMMUNOCYTOCHEMISTRY AND CONFOCAL LASER MICROSCOPY. Morphology, 2018, 153(1): 71-75 DOI:10.17816/morph.398199

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Гурин Я. В. Морфологический анализ новообразованных сосудов и клеточного микроокружения // Бюл. экспер. биол. 2009. Т. 147, № 11. С. 593-596.

[2]	Гурина О. Ю., Куприянов В. В., Миронов А. А., Миронов В. А. Механизмы неоваскулогенеза и его регуляции во взрослом организме // Арх. анат. 1985. Т. 88, № 1. С. 9-24.

[3]	Коржевский Д. Э., Отеллин В. А., Неокесарийский А.А, Старорусская А. Н., Павлова Н. Г. Организация и цитохимические особенности барьерных структур плаценты человека // Морфология. 2006. Т. 129, вып. 3. С. 63-64.

[4]

Коржевский Д. Э., Сухорукова Е. Г., Гилерович Е. Г., Петрова Е. С., Кирик О. В., Григорьев И. П. Преимущества и недостатки цинк-этанол-формальдегида как фиксатора для иммуноцитохимических исследований и конфокальной лазерной микроскопии // Морфология. 2013. Т. 143, вып. 2. С. 81-85. Переводная версия: Korzhevskii D. E., Sukhorukova E. G., Gilerovich E. G., Petrova E. S., Kirik O. V., Grigor’ev I. P. Advantages and disadvantages of zinc-ethanolformaldehyde as a fixative for immunocytochemical studies and confocal laser microscopy // Neurosci Behav Physiol 2014. Vol. 44, № 5. P. 542-545. doi: 10.1007/s11055-014-9948-8.

[5]	Павлович Е. Р., Гурин Я. В., Гурина О. Ю. Ультраструктура телец Вейбеля-Палада в эндотелиоцитах сосудистого русла млекопитающих разных видов // Успехи современного естествознания. 2010. № 9. С. 123-125.

[6]	Jazayeri M., Allameh A., Soleimani M. et al. Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells // Cell Biol. Int. 2008. Vol. 32, № 10. P. 1183-1192. doi: 10.1016/j. cellbi.2008.07.020.

[7]	Lopes da Silva M., Cutler D. F. von Willebrand factor multimerization and the polarity of secretory pathways in endothelial cells // Blood. 2016. Vol. 128, № 2. P. 277-285. doi: 10.1182/ blood-2015-10-677054.

[8]	Nightingale T., Cutler D. The secretion of von Willebrand factor from endothelial cells; an increasingly complicated story // J. Thromb. Haemost. 2013. Vol. 11, Suppl. 1. P. 192-201. doi: 10.1111/jth.12225.

[9]	Peyvandi F., Garagiola I., Baronciani L. Role of von Willebrand factor in the haemostasis // Blood Transfus. 2011. Vol. 9, Suppl. 2. P. 3-8. doi: 10.2450/2011.002S.

[10]	Starke R. D., Ferraro F., Paschalaki K. E. et al. Endothelial von Willebrand factor regulates angiogenesis // Blood. 2011. Vol. 117, № 3. P. 1071-1080. doi: 10.1182/blood-2010-01-264507.

[11]	Trotman W. E., Taatjes D. J., Bovill E. G. Multifluorescence confocal microscopy: application for a quantitative analysis of hemostatic proteins in human venous valves // Methods Mol. Biol. 2013. Vol. 931. P. 85-95. doi: 10.1007/978-1-62703-056-4_4.

[12]	Wagner D. D., Bonfanti R. von Willebrand factor and the endothelium // Mayo Clin. Proc. 1991. Vol. 66, № 6. P. 621-627.

[13]	Weibel E., Palade G. New cytoplasmic components in arterial endothelia // J. Cell Biol. 1964. Vol. 23. P. 101-112.

[14]	Wu X. X., Gordon R. E., Glanville R. W. et al. Morphological relationships of von Willebrand factor, type VI collagen, and fibrillin in human vascular subendothelium // Am. J. Pathol. 1996. Vol. 149, № 1. P. 283-291.