CYTOARCHITECTURE OF THE SENSORIMOTOR CORTEX AND CA1 FIELD OF THE HIPPOCAMPUS IN THE BRAIN OF ALBINO RATS WITH DIFFERENT SEIZURE ACTIVITY IN POST-TRAUMATIC PERIOD

Abstract

Objective - to study the cytoarchitecture of the sensorimotor cortex (SMC) and CA1 field of the hippocampus of albino rat brain after severe traumatic brain injury. Material and methods . The polytrauma was modeled according to Noble-Collip method under the anesthesia. The main group (n=25) included animals with convulsive paroxysms (CP) and the comparison group (n=25) - rats free of them. The material was collected on days 1, 3, 7, 14 and 30 after the injury. The brain sections were stained with Nissl method, total numerical density of pyramidal neurons (TNDN) and the proportion of dark neurons (DN) were determined. Verification of statistical hypotheses was performed using Statistica 8.0. Results . One day after trauma, the number of DN was significantly increased in SMC and CA1 field of the hippocampus. In the late recovery period, in the comparison group 65.0% of DN in SMC restored to normochromic cells, while in CA1 field of the hippocampus this was found in 18% neurons. The emergence of CP prevented the restoration of DN: in SMC 54.5% of the neurons, and in CA1 field of the hippocampus - only 2.7% of the cells were restored. As a result, in the main group TNDN in SMC decreased by 32.4%, while in CA1 field of the hippocampus it was reduced by 55.6% and in the comparison group - by 24.7% and 43.3%, respectively. Conclusions. CP are a factor contributing to the pathological changes of neurons in the post-traumatic period.

Keywords

brain / neocortex / hippocampus / cytoarchitecture / traumatic brain injury

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