TYPE I COLLAGEN, SIRTUIN-6, AND MATRIX METALLOPROTEINASE-1 EXPRESSION IN HUMAN SKIN FIBROBLASTS DURING LONG-TERM CULTIVATION

N. V. Fridman; N. S. Lin’kova; V. O. Polyakova; A. O. Drobintseva; S. V. Trofimova; A. V. Dudkov; V. Kh. Khavinson; I. M. Kvetnoy

doi:10.17816/morph.398186

Morphology ›› 2018, Vol. 153 ›› Issue (1) : 39 -44. DOI: 10.17816/morph.398186

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TYPE I COLLAGEN, SIRTUIN-6, AND MATRIX METALLOPROTEINASE-1 EXPRESSION IN HUMAN SKIN FIBROBLASTS DURING LONG-TERM CULTIVATION

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St. Petersburg Bioregulation and Gerontology Institute

Peter the Great St. Petersburg Polytechnic University

D. O. Ott Scientific-Research Institute of Obstetrics, Gynecology and Reproduction

St. Petersburg State University

St Petersburg State Pediatric Medical University

RAS I. P. Pavlov Institute of Physiology

Department of Biogerontology, Department of Cell Biology and Pathology

Department of Biogerontology, Department of Cell Biology and Pathology; Department of Medical Physics; Institute of Physics, Nanotechnology and Telecommunications

Department of Pathomorphology; Departments of Pathology and Physiology

Department of Pathomorphology; Department of Medical Biology

Department of Biogerontology, Department of Cell Biology and Pathology

Department of Biogerontology

Department of Biogerontology; Group of Peptide Regulation of Ageing

Department of Biogerontology; Department of Pathomorphology; Departments of Pathology and Physiology

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Abstract

Objective - to verify the senescence markers of skin fibroblasts in in vitro model. Material and methods. The study was performed on cultured human skin fibroblasts of 3rd passage (“young» cultures) and 14th passage (“old” cultures). The expression of type I collagen, sirtuin-6 and matrix mettaloproteinase-1 (MMP-1) in skin fibroblasts cultures was examined using the method of immunofluorescence confocal microscopy. Results . It was shown that during cell aging, the expression of type I collagen and sirtuin-6 in skin fibroblasts cultures decreased 3.5 and 3.6 times respectively, while the expression of MMP-1 increased 2.5 times. Conclusions . It is suggested, that decreasing sirtuinn-6 expression results in the disturbances of type I collagen synthesis and increase in MMP-1 expression. This mechanism can play an important role in the understanding of molecular mechanisms of skin aging.

Keywords

human skin fibroblasts / aging / sirtuin-6 / type I collagen / matrix metalloproteinase

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N. V. Fridman, N. S. Lin’kova, V. O. Polyakova, A. O. Drobintseva, S. V. Trofimova, A. V. Dudkov, V. Kh. Khavinson, I. M. Kvetnoy. TYPE I COLLAGEN, SIRTUIN-6, AND MATRIX METALLOPROTEINASE-1 EXPRESSION IN HUMAN SKIN FIBROBLASTS DURING LONG-TERM CULTIVATION. Morphology, 2018, 153(1): 39-44 DOI:10.17816/morph.398186

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