TYPE I COLLAGEN, SIRTUIN-6, AND MATRIX METALLOPROTEINASE-1 EXPRESSION IN HUMAN SKIN FIBROBLASTS DURING LONG-TERM CULTIVATION
N. V. Fridman , N. S. Lin’kova , V. O. Polyakova , A. O. Drobintseva , S. V. Trofimova , A. V. Dudkov , V. Kh. Khavinson , I. M. Kvetnoy
Morphology ›› 2018, Vol. 153 ›› Issue (1) : 39 -44.
TYPE I COLLAGEN, SIRTUIN-6, AND MATRIX METALLOPROTEINASE-1 EXPRESSION IN HUMAN SKIN FIBROBLASTS DURING LONG-TERM CULTIVATION
Objective - to verify the senescence markers of skin fibroblasts in in vitro model. Material and methods. The study was performed on cultured human skin fibroblasts of 3rd passage (“young» cultures) and 14th passage (“old” cultures). The expression of type I collagen, sirtuin-6 and matrix mettaloproteinase-1 (MMP-1) in skin fibroblasts cultures was examined using the method of immunofluorescence confocal microscopy. Results . It was shown that during cell aging, the expression of type I collagen and sirtuin-6 in skin fibroblasts cultures decreased 3.5 and 3.6 times respectively, while the expression of MMP-1 increased 2.5 times. Conclusions . It is suggested, that decreasing sirtuinn-6 expression results in the disturbances of type I collagen synthesis and increase in MMP-1 expression. This mechanism can play an important role in the understanding of molecular mechanisms of skin aging.
human skin fibroblasts / aging / sirtuin-6 / type I collagen / matrix metalloproteinase
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