GLUTAMINE SYNTHETASE IN RAT BRAIN CELLS

Ye. G. Sukhorukova; V. V Gusel’nikova; D. E. Korzhevskiy

doi:10.17816/morph.398174

Morphology ›› 2017, Vol. 152 ›› Issue (6) : 7 -10. DOI: 10.17816/morph.398174

Articles

research-article

GLUTAMINE SYNTHETASE IN RAT BRAIN CELLS

Author information +

History +

PDF

Abstract

Objective - to study, using immunohistochemical method, the cells in the brain producing glutamine synthetase (GS). Material and methods. The enzyme was demonstrated in the frontal sections of rat brain (n=10) using monoclonal mouse antibody. The preparations were analyzed using light and confocal laser microscopy. Results. The study showed ubiquitous expression of GS in the brain mainly by two morphologically and topographically different cell types. According to their structure and localization, as well as the immunopositive reaction to glial fibrillary acidic protein, the dominant type of cells was identified as astrocytes. Cells of another type had morphological characteristics and localization corresponding to those of typical oligodendrocytes. Conclusions. Thus, the data obtained suggest that GS is not a selective marker of any cell population of the rat brain.

Keywords

brain / glia / glutamine synthetase / immunohistochemistry

Cite this article

Download citation ▾

Ye. G. Sukhorukova, V. V Gusel’nikova, D. E. Korzhevskiy. GLUTAMINE SYNTHETASE IN RAT BRAIN CELLS. Morphology, 2017, 152(6): 7-10 DOI:10.17816/morph.398174

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Коржевский Д. Э., Сухорукова Е. Г., Гилерович Е. Г., Петрова Е. С., Кирик О. В., Григорьев И. П. Преимущества и недостатки цинк-этанол-формальдегида как фиксатора для иммуноцитохимических исследований и конфокальной лазерной микроскопии // Морфология. 2013. Т. 143, № 2. С. 81-85.

[2]	Сухорукова Е. Г., Коржевский Д. Э., Алексеева О. С. Глиальный фибриллярный кислый белок - компонент промежуточных филаментов астроцитов мозга позвоночных // Журн. эволюц. биохим. 2015. Т. 51, № 1. С. 3-10.

[3]	Anlauf E., Derouiche A. Glutamine synthetase as an astrocytic marker: its cell type and vesicle localization // Front Endocrinol. (Lausanne). 2013. Vol. 4, № 144. P. 1-5.

[4]	Bak L. K., Schousboe A., Vaagepetersen H. S. The glutamate/ GABA-glutamine cycle: aspects of transport, neurotransmitter homeostasis and ammonia transfer // J. Neurochem. 2006. Vol. 98, № 3. P. 641-653.

[5]	Bernstein H.-G., Bannier J., Meyer-Lotz G. et al. Distribution of immunoreactive glutamine synthetase in the adult human and mouse brain. Qualitative and quantitative observations with special emphasis on extra-astroglial protein localization // J. Chemical Neuroanat. 2014. Vol. 61-62. P. 33-50.

[6]	Cammer W. Glutamine synthetase in the central nervous system is not confined to astrocytes // J. Neuroimmunol. 1990. Vol. 26. P. 173-178.

[7]	D’Amelio F., Eng L. F., Gibbs M. A. Glutamine synthetase immunoreactivity is present in oligodendroglia of various regions of the central nervous system // Glia. 1990. Vol. 3, № 5. P. 335-341.

[8]	Fernandes S. P., Dringen R., Lawen A., Robinson S. R. Neurones express glutamine synthetase when deprived of glutamine or interaction with astrocytes // J. Neurochem. 2010. Vol. 114, № 5. P. 1527-1536.

[9]	Hertz L., Zielke H. R. Astrocytic control of glutamatergic activity: astrocytes as stars of the show // Trends Neurosci. 2004. Vol. 27, № 12. P. 735-743.

[10]	Korzhevskii D. E., Sukhorukova E. G., Kirik O. V., Grigorev I. P. Immu nohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde // Europ J. Histochem. 2015. Vol. 59, № 3. P. 5-9.

[11]	Norenberg M. D., Martinez-Hernandez A. Fine structural localization of glutamine synthetase in astrocytes of rat brain // Brain Res. 1979. Vol. 161, № 2. P. 303-310.

[12]	Rose C. F., Verkhratsky A., Parpura V. Astrocyte glutamine synthetase: pivotal in health and disease // Biochem Soc Trans. 2013. Vol. 41, № 6. P. 1518-1524.