RAPID METHOD OF THE AMYLOID STAINING WITH CONGO RED FOR LIGHT AND FLUORESCENCE MICROSCOPY

V. V. Gusel’nikova; O. V. Kirik; Ye. A. Fyodorova; M. M. Shavlovskiy; A. Ya. Gudkova; D. E. Korzhevskiy

doi:10.17816/morph.397669

Morphology ›› 2016, Vol. 149 ›› Issue (2) : 84 -88. DOI: 10.17816/morph.397669

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RAPID METHOD OF THE AMYLOID STAINING WITH CONGO RED FOR LIGHT AND FLUORESCENCE MICROSCOPY

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RAS Research Institute of Experimental Medicine

I. P.Pavlov First St. Petersburg State Medical University

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology; Laboratory of Cardiomyopathies, Institute of Cardiovascular Diseases

Laboratory of Cardiomyopathies, Institute of Cardiovascular Diseases

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology; Laboratory of Cardiomyopathies, Institute of Cardiovascular Diseases

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Abstract

The aim of the present study was to optimize the histochemical method of amyloid staining using Congo red. The study was performed on specimens of the myocardium of left ventricle of the heart obtained at autopsy from the patients with amyloidosis of myocardium diagnosed postmortem. It was shown that a positive impact on the quality of the staining of amyloid is provided by a procedure of pre-heating the slides in the liquid, especially at an acidic pH. The staining protocol was developed allowing to obtain preparations characterized by high-contrast staining of amyloid at light microscopic level and by high intensity of its fluorescence. The advantage of the protocol presented is also a significant reduction in the total duration of staining, an increase in stability of the specific staining of amyloid and the absence of nonspecific background staining.

Keywords

amyloid / Congo red / histochemistry / fluorescence microscopy

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V. V. Gusel’nikova, O. V. Kirik, Ye. A. Fyodorova, M. M. Shavlovskiy, A. Ya. Gudkova, D. E. Korzhevskiy. RAPID METHOD OF THE AMYLOID STAINING WITH CONGO RED FOR LIGHT AND FLUORESCENCE MICROSCOPY. Morphology, 2016, 149(2): 84-88 DOI:10.17816/morph.397669

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References

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