CURRENT HISTOCHEMICAL METHODS OF TISSUE IRON DEMONSTRATION BASED ON PERLS’ REACTION
I. P. Grigoriyev , Ye. A. Kolos , Ye. G. Sukhorukova , D. E. Korzhevskiy
Morphology ›› 2016, Vol. 149 ›› Issue (1) : 85 -88.
CURRENT HISTOCHEMICAL METHODS OF TISSUE IRON DEMONSTRATION BASED ON PERLS’ REACTION
The article presents the information on the modern modifications of Perls’ reaction including diaminobenzidine enhancement according to R. Meguro (for various tissues), M. A. Smith (for nervous tissue), S. M. Levine (for detection of iron in oligodendrocytes and myelinated nervous fibers), and our own modification for visualization of the nucleolar iron. The analysis is performed of the advantages and disadvantages of these modifications of histochemical demonstration of iron. It is shown that the use of new methodological approaches significantly increases the sensitivity of Perls’ reaction. Control procedures allow to to eliminate the possibility of artifacts.
iron / histochemistry / Perls’ reaction / nervous tissue / nucleolus
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