LOCALIZATION AND QUANTITATIVE ASSESSMENT OF OXYGEN-DEPENDENT HYPOXIA-INDUCIBLE FACTOR 1α IN THE BRAIN OF MITTEN CRAB ERIOCHEIR JAPONICA IN NORM AND IN ACUTE ANOXIA (AN IMMUNOHISTOCHEMICAL STUDY)

Abstract

Using immunoblotting and immunocytochemistry, the expression of hypoxia-inducible factor 1α (HIF-1α) was studied in the brain of the mitten crab Eriocheir japonica in norm and at 2, 4, 6 and 12 hours of anoxia on the model of water deprivation. In intact crabs, the number of immunopositive neurons was small, but it increased with anoxia duration. Particularly pronounced increase in the proportion of neurons with the HIF-1α expression was found in cell group 6. In group 9/11, the highest expression index was observed between 2-6 hours of anoxia. In group 17, significant changes in the proportion of immunopositive cells was observed only after 2 hours of anoxia. After 6 hours of anoxia, proportion of neurons with HIF-1α expression within all cell groups was reduced, but the reactions appeared in the blood cells. It is assumed that the increase in the proportion of immunopositive neurons and the appearance of the expression of HIF-1α in blood cells in the anoxic brain play an important role in providing compensatory and protective processes, enhancing adaptive capacity of mitten crab under the conditions of hypoxic stress.

Keywords

central nervous system / the mitten crab / hypoxia-inducible factor 1α / anoxia

References

[1] Beltz B. S., Zhang Y., Benton J. L., Sandeman D. C. Adult neurogenesis in the decapod crustacean brain: a hematopoietic connection? // Eur. J. Neurosci. 2011. Vol. 34. P. 870-883.

[2] Benton J. L., Kery R., Li J. et al. Cells from the immune system generate adult-born neurons in crayfish // Dev. Cell. 2014. Vol. 30, № 3. P. 322-333.

[3] Bickler P. E., Donohoe P. H., Buck L. T. Molecular adaptations for survival during anoxia: lessons from lower vertebrates // Neuroscience. 2002. Vol. 8. P. 234-242.

[4] Bruick R. K. Oxygen sensing in the hypoxic response pathway, regulation of the hypoxia-inducible transcription factor // Genes Dev. 2003. Vol. 17. P. 2614-2623.

[5] Frenkel L., Maldonado H., Delorenzi A. Memory strengthening by a real-life episode during reconsolidation: an outcome of water deprivation via brain angiotensin II // Eur. J. Neurosci. 2005. Vol. 22. P. 1757-1766.

[6] Gorr T. A.,Wichmann D., Hu J. et al. Hypoxia tolerance in animals: biology and application // Physiol. Biochem. Zool. 2010. Vol. 83. P. 733-742.

[7] Hardy K. M., Follett C. R., Burnett L. E., Lema S. C. Gene transcripts encoding hypoxia-inducible factor (HIF) exhibit tissue-and muscle fiber type-dependent responses to hypoxia and hypercapnic hypoxia in the Atlantic blue crab, Callinectes sapidus // Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2012. Vol. 163, № 1. P. 137-146.

[8] Kawabe S., Yokoyama Y. Role of Hypoxia-inducible factor α in response to hypoxia and heat shock in the pacific oyster Crassostrea gigas // Mar. Biotechnol. 2012. Vol. 14. P. 106-119.

[9] Kodama K., Rahman M. S., Horiguchi T., Thomas P.Assessment of hypoxia-inducible factor-1α mRNA expression in mantis shrimp as a biomarker of environmental hypoxia exposure // Biol. Lett. 2012. Vol. 8, № 2. P. 278-281.

[10] Li T., Brouwer M. Hypoxia-inducible factor, gsHIF, of the grass shrimp Palaemonetes pugio: molecular characterization and response to hypoxia // Comp. Biochem. Physiol. 2007. Vol. 147. P. 11-19.

[11] López-Hernández B., Posadas I., Podlesniy P. et al. HIF-1α is neuroprotective during the early phases of mild hypoxia in rat cortical neurons // Exp. Neurol. 2012. Vol. 233, № 1. P. 543-544.

[12] Piontkivska H., Chung J. S., Ivanina, A. V. et al. Molecular cha racterization and mRNA expression of two key enzymes of hypoxia-sensing pathways in eastern oysters Crassostrea virginica (Gmelin): hypoxia-inducible factor α (HIF-α) and HIF-prolyl hyd roxylase (PHD) // Comp. Biochem. Physiol. 2011. Vol. 6. P. 103-114.

[13] Sandeman D. C., Sandeman R. E., Derby C., Schmidt M. Morphology of the brain of crayfish, crabs, and spiny lobsters: a common nomenclature for homologous structures // Biol. Bull. 1992. Vol. 183. P. 304-326.

[14] Schmidt M., Derby C. D. Cytoarchitecture and ultrastructure of neural stem cell niches and neurogenic complexes maintaining adult neurogenesis in the olfactory midbrain of spiny lobsters, Panulirus argus // Comp. Neurol. 2011. Vol. 519, № 12. P. 2283- 2319.

[15] Sharp F. R., Bernaudin M. HIF-1 and oxygen sensing in the brain // Nat. Rev. Neurosci. 2004. Vol. 5. P. 437-448.

[16] Shirinyan D., Teshiba T., Taylor K. et al. Rostral ganglia are re quired for induction but not expression of crayfish escape reflex habituation: role of higher centers in reprogramming low-level circuits // J. Neurophysiol. 2006. Vol. 95. P. 2721-2724.

[17] Soñanez-Organis J. G., Peregrino-Uriarte A. B., Gómez-Jiménez S. et al. Molecular characterization of hypoxia inducible factor-1 (HIF-1) from the white shrimp Litopenaeus vannamei and tissue specific expression under hypoxia // Comp. Biochem. Physiol. 2009. Vol. 150. P. 395-405.

[18] Vangeison G., Carr D., Federoff H. J., Rempe D. A. The good, the bad, and the cell type-specific roles of hypoxia inducible factor-1 alpha in neurons and astrocytes // J. Neurosci. 2008. Vol. 28. P. 1988-1993.

[19] Zarember K. A., Malech H. L. HIF-1alpha: a master regulator of innate host defenses? // J. Clin. Invest. 2005. Vol. 115. P. 1702-1704.

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