The analysis of histological features and morphometric parameters of the ovaries in absenсe epilepsy

Abstract

BACKGROUND: Absence epilepsy is one of the most common epileptic syndromes and is more common in girls. Often in adolescence, generalized tonic-clonic seizures (up to 50%) join. In women suffering from epilepsy, reproductive dysfunction is often noted. In this regard, it seems relevant to study the morphofunctional state of the ovaries in absence epilepsy.

AIM: Assessment of morphological and morphometric parameters of the ovaries in absence epilepsy.

MATERIALS AND METODS: Using histological and morphometric methods, the ovaries of Wistar and WAG/Rij rats (a genetic model of human absence epilepsy) were studied.

RESULTS: In WAG/Rij rats, destruction of the cortical and medulla of the ovary was found: the layer of granulosa cells surrounding the oocyte had a looser structure compared to the norm, destructive changes in the follicular epithelium and apoptotic death of granulosa cells were observed. Morphometric analysis showed that in WAG/Rij rats the weight of the ovaries was 2 times less than in Wistar rats: 37.30±0.93 mg versus 55.84±2.36 mg. The thickness of the epithelial layer was 7.81±0.25 μm, which is 26.2% less than in Wistar rats (p <0.05). The ovarian follicles of WAG/Rij rats were irregular in shape, smaller in diameter and area, and the numerical density of all types of follicles was lower compared to Wistar rats, which indicates a violation of ovulation processes.

CONCLUSION: Structural and quantitative changes in the ovaries of WAG/Rij rats were observed in absence epilepsy, which indicates violations of the endocrine function of the ovaries and process of folliculogenesis.

Keywords

WAG/Rij rats / absence epilepsy / ovaries / follicles / reproductive system

References

[1] Isojärvi JI. Reproductive dysfunction in women with epilepsy. Neurology. 2003;61(6 (suppl. 2). P. 27–34. doi: 10.1212/wnl.61.6_suppl_2.s27

[2] Isojärvi J.I. Reproductive dysfunction in women with epilepsy // Neurology. 2003. Vol. 61, N 6 (suppl. 2). P. 27–34. doi: 10.1212/wnl.61.6_suppl_2.s27

[3] Markoula S, Siarava E, Keramida A, et al. Reproductive health in patients with epilepsy. Epilepsy Behav. 2020;113:107563. doi: 10.1016/j.yebeh.2020.107563

[4] Markoula S., Siarava E., Keramida A., et al. Reproductive health in patients with epilepsy // Epilepsy Behav. 2020. Vol. 113. P. 107563. doi: 10.1016/j.yebeh.2020.107563

[5] Nakamura J, Sorge ST, Winawer MR, et al. Reproductive decision-making in families containing multiple individuals with epilepsy. Epilepsia. 2021;6(5):1220–1230. doi: 10.1111/epi.16889

[6] Nakamura J., Sorge S.T., Winawer M.R., et al. Reproductive decision-making in families containing multiple individuals with epilepsy // Epilepsia. 2021. Vol. 6, N 5. P. 1220–1230. doi: 10.1111/epi.16889

[7] Santos AMC, Castro Lima Filho H, Siquara GM, et al. Sexual function in women of fertile age with epilepsy. Epilepsy Behav. 2021;125:108399. doi: 10.1016/j.yebeh.2021.108399

[8] Santos A.M.C., Castro Lima Filho H., Siquara G.M., et al. Sexual function in women of fertile age with epilepsy // Epilepsy Behav. 2021. Vol. 125. P. 108399. doi: 10.1016/j.yebeh.2021.108399

[9] Sadrtdinova I, Khismatullina Z. Amygdala in the regulation of reproductive functions during absence epilepsy. Мoscow: Nauchno-izdatel’skij centr «INFRA-M»; 2018. 148 p. (In Russ).

[10] Садртдинова И.И., Хисматуллина З.Р. Амигдала в системе регуляции репродуктивных функций организма при абсансной эпилепсии. Москва : Научно-издательский центр «ИНФРА-М», 2018. 148 с. doi: 10.12737/monography_59fc4bd4451037.74493919

[11] Verrotti A, Greco R, Latini G, Chiarelli F. Endocrine and metabolic changes in epileptic patients receiving valproic acid. J Pediatr Endocrinol Metab. 2005;18(5):423–430. doi: 10.1515/jpem.2005.18.5.423

[12] Verrotti A., Greco R., Latini G., Chiarelli F. Endocrine and metabolic changes in epileptic patients receiving valproic acid // J Pediatr Endocrinol Metab. 2005. Vol. 18, N 5. P. 423–430. doi: 10.1515/jpem.2005.18.5.423

[13] Kotov AS. Jepilepsija u zhenshhin reproduktivnogo vozrasta. Annals of Clinical and Experimental Neurology. 2010;4(3):10–13. (In Russ).

[14] Котов А.С. Эпилепсия у женщин репродуктивного возраста // Анналы клинической и экспериментальной неврологии. 2010. Т. 4, № 3. С. 10–13.

[15] Genton P, Bauer J, Duncan S, et al. On the association between valproate and polycystic ovary syndrome. Epilepsia. 2001;42(3):295–304. doi: 10.1046/j.1528-1157.2001.28899.x

[16] Genton P., Bauer J., Duncan S., et al. On the association between valproate and polycystic ovary syndrome // Epilepsia. 2001. Vol. 42, N 3. P. 295–304. doi: 10.1046/j.1528-1157.2001.28899.x

[17] Bilo L, Meo R, Valentino R, et al. Characterization of reproductive endocrine disorders in women with epilepsy. J Clin Endocrinol Metab. 2001;86(7):2950–2956. doi: 10.1210/jcem.86.7.7633

[18] Bilo L., Meo R., Valentino R., et al. Characterization of reproductive endocrine disorders in women with epilepsy // J Clin Endocrinol Metab. 2001. Vol. 86, N 7. P. 2950–2956. doi: 10.1210/jcem.86.7.7633

[19] Pack A. Effects of treatment on endocrine function in patients with epilepsy. Curr Treat Options Neurol. 2005;7(4):273–280. doi: 10.1007/s11940-005-0037-8

[20] Pack A. Effects of treatment on endocrine function in patients with epilepsy // Curr Treat Options Neurol. 2005. Vol. 7, N 4. P. 273–280. doi: 10.1007/s11940-005-0037-8

[21] Cramer JA, Jones EE. Reproductive function in epilepsy. Epilepsia. 1991;32 suppl. 6:S19–S26. doi: 10.1111/j.1528-1157.1991.tb05887.x

[22] Cramer J.A., Jones E.E. Reproductive function in epilepsy // Epilepsia. 1991. Vol. 32, N 6. P. 19–26. doi: 10.1111/j.1528-1157.1991.tb05887.x

[23] Bauer J, Cooper-Mahkorn D. Reproductive dysfunction in women with epilepsy: menstrual cycle abnormalities, fertility, and polycystic ovary syndrome. Int Rev Neurobiol. 2008;83:135–155. doi: 10.1016/S0074-7742(08)00007-X

[24] Bauer J., Cooper-Mahkorn D. Reproductive dysfunction in women with epilepsy: menstrual cycle abnormalities, fertility, and polycystic ovary syndrome // Int Rev Neurobiol. 2008. Vol. 83, P. 135–155. doi: 10.1016/S0074-7742(08)00007-X

[25] Markoula S, Siarava E, Keramida A, et al. Reproductive health in patients with epilepsy. Epilepsy Behav. 2020 Dec;113:107563. doi: 10.1016/j.yebeh.2020.107563

[26] Markoula S., Siarava E., Keramida A., et al. Reproductive health in patients with epilepsy// Epilepsy Behav. 2020. Vol. 113, P. 107563. doi: 10.1016/j.yebeh.2020.107563

[27] Emori C, Sugiura K. Role of oocyte-derived paracrine factors in follicular development. Anim Sci J. 2014;85(6):627–633. doi: 10.1111/asj.12200

[28] Emori C., Sugiura K. Role of oocyte-derived paracrine factors in follicular development // Anim Sci J. 2014.Vol. 85, N 6. Р. 627–633. doi: 10.1111/asj.12200

[29] Sinowatz F, Kölle S, Töpfer-Petersen E. Biosynthesis and expression of zonapellucida glycoproteins in mammals. Cells Tissues Organs. 2001;168(1–2):24–35. doi: 10.1159/000016803

[30] Sinowatz F., Kölle S., Töpfer-Petersen E. Biosynthesis and expression of zonapellucida glycoproteins in mammals // Cells Tissues Organs. 2001. Vol. 168, N 1–2. P. 24–35. doi: 10.1159/000016803

[31] Avella MA, Xiong B, Dean J. The molecular basis of gamete recognition in mice and humans Mol Hum Reprod. 2013;19(5):279–289. doi: 10.1093/molehr/gat004

[32] Avella M.A., Xiong B., Dean J. The molecular basis of gamete recognition in mice and humans // Mol Hum Reprod. 2013. Vol. 19, N 5. P. 279–289. doi: 10.1093/molehr/gat004

[33] Sein OB, Sein DO, Kononova MS. Osobennosti follikulogeneza u svinej v period pubertata. Bulletin of the Kursk State Agricultural Academy. 2010;(1):76–78. (In Russ).

[34] Сеин О.Б., Сеин Д.О., Кононова М.С. Особенности фолликулогенеза у свиней в период пубертата // Вестник Курской государственной сельскохозяйственной академии. 2010. № 1. С. 76–78.

[35] Fawley JA, Pouliot WA, Dudek FE. Epilepsy and reproductive disorders: the role of the gonadotropin-releasing hormone network. Epilepsy Behav. 2006;8(3):477–482. doi: 10.1016/j.yebeh.2006.01.019

[36] Fawley J.A., Pouliot W.A., Dudek F.E. Epilepsy and reproductive disorders: the role of the gonadotropin-releasing hormone network // Epilepsy Behav. 2006. Vol. 8, N 3. P. 477–482. doi: 10.1016/j.yebeh.2006.01.019

[37] Kulakov VI, Ovsjannikova TV. Problemy i perspektivy lechenija besplodija v brake. Obstetrics and Gynecology. 1997;(3):5–8. (In Russ).

[38] Кулаков В.И., Овсянникова Т.В. Проблемы и перспективы лечения бесплодия в браке // Акушерство и гинекология. 1997. № 3. С. 5–8.

[39] Dindjaev S.V. Funkcional’naja i klinicheskaja morfologija jaichnikov. Ivanovo: Ivanovskaja gosudarstvennaja medicinskaja akademija; 2010. 218. (In Russ).

[40] Диндяев С.В. Функциональная и клиническая морфология яичников. Иваново : Ивановская государственная медицинская академия, 2010. 218 с.

[41] Islam MR, Ichii O, Nakamura T, et al. Unique morphological characteristics in the ovary of cotton rat (Sigmodon hispidus). J Reprod Dev. 2020;66(6):529–538. doi: 10.1262/jrd.2020-061

[42] Islam M.R., Ichii O., Nakamura T., et al. Unique morphological characteristics in the ovary of cotton rat (Sigmodon hispidus) // J Reprod Dev. 2020. Vol. 66, N 6. P. 529–538. doi: 10.1262/jrd.2020-061

[43] Dundekova N, Shubina O, Komarova N. The morphological features of the ovaries during lead intoxication. Estestvennye i matematicheskie nauki v sovremennom mire. 2015;34:67–81. (In Russ).

[44] Дуденкова Н.А., Шубина О.С., Комарова Н.А. Морфологические особенности строения яичников при свинцовой интоксикации // Естественные и математические науки в современном мире. 2015. № 34. С. 67–81.

[45] Verrotti A, D’Egidio C, Mohn A, et al. Antiepileptic drugs, sex hormones, and PCOS. Epilepsia. 2011;52(2):199–211. doi: 10.1111/j.1528-1167.2010.02897.x

[46] Verrotti A., D’Egidio C., Mohn A., et al. Antiepileptic drugs, sex hormones, and PCOS // Epilepsia. 2011. Vol. 52, N 2. P. 199–211. doi: 10.1111/j.1528-1167.2010.02897.x

[47] Morrell MJ, Guidice L, Flynn KL, et al. Predictors of ovulatiry failure in women with epilepsy. Ann Neurol. 2002;52(6):704–711. doi: 10.1002/ana.10391

[48] Morrell M.J., Guidice L., Flynn K.L., et al. Predictors of ovulatiry failure in women with epilepsy // Ann Neurol. 2002. V. 52, N 6. P. 704–11. doi: 10.1002/ana.10391

[49] Herzog AG, Coleman AE, Jacobs AR, et al. Interictal EEG discharges, reproductive hormones, and menstrual disorders in epilepsy. Ann Neurol. 2003;54(5):625–637. doi: 10.1002/ana.10732

[50] Herzog A.G., Coleman A.E., Jacobs A.R., et al. Interictal EEG discharges, reproductive hormones, and menstrual disorders in epilepsy // Ann Neurol. 2003. Vol. 54, N 5. P. 625–637. doi: 10.1002/ana.10732

[51] Rakhmatullina GA, Sadrtdinova II. effect of vitamin E on the estrous cycle of WAG/RIJ rats. Doklady Bashkirskogo universiteta. 2020;5(2):88–91. (In Russ). doi: 10.33184/dokbsu-2020.2.2

[52] Рахматуллина Г.А., Садртдинова И.И. Влияние витамина Е на эстральный цикл крыс линии WAG/Rij // Доклады Башкирского университета. 2020. Т. 5, № 2. С. 88–91. doi 10.33184/dokbsu-2020.2.2

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