METHOD FOR VISUALIZATION OF BRAIN HISTAMINERGIC NEURONS IN HYPOTHALAMUS

Abstract

Objective - the development of immunohistochemical method for visualization of brain histaminergic neurons in the rat hypothalamus. Material and methods. The study was carried out on the samples of hypothalamus of 10 outbred albino male rats aged 45-90 days. For the selective detection of histaminergic neurons on paraffin sections of the hypothalamus, an immunohistochemical reaction was performed with anti-monoamine oxidase type B antibodies. Results. The method was described for the selective detection of histaminergic neurons in the hypothalamus on the paraffin sections of hypothalamus by immunohistochemical reaction with anti-monoamine oxidase type B antibodies. Conclusions. The method allows to visualize histaminergic neurons on paraffin sections of the hypothalamus.

Keywords

histaminergic neurons / hypothalamus / rat brain / monoamine oxidase B

RIGHTS & PERMISSIONS

Zimatkin S.M., Zaerko A.V.