EXPERIMENTAL BASIS OF THE USE OF POLYCAPROLACTONE-BASED SCAFFOLDS MINERALIZED WITH VATERITE FOR STIMULATION OF BONE TISSUE REGENERATION

Abstract

Objective - to investigate the regeneration of bone tissue in albino rats after bone defect replacement with a scaffold made of polycaprolactone (PCL) and vaterite (CaCO3). Material and methods. The experiment was carried out on 20 outbred albino rats. The PCL-scaffolds with a foreign protein were implanted into the femoral defect area in animals of the control group (n=10), and PCL/CaCO3-scaffolds were implanted into the rats of the experimental group (n=10). The evaluation of reparative processes was performed using microscopy of the hematoxylin- and eosin-stained femoral diaphysis cross-sections. Results. In animals of the control group 28 days after implantation the scaffold was separated by a connective and bone tissue, no signs of osteogenesis were detected in the matrix. In animals of the experimental group, the formation of bone trabeculae was observed in the matrix due to its osteoinductive properties. Conclusion. PLC/CaCO3-scaffolds are biocompatible, integrate well into bone tissue of experimental animals, and have pronounced osteoinductive effect. The prospects of clinical approbation of this type of matrix for the stimulation of bone tissue regeneration in traumatological and orthopedic patients have been experimentally substantiated.

Keywords

bone tissue / regeneration / lower limbs / biocompatibility / scaffolds / vaterite / polycaprolactone

References

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RIGHTS & PERMISSIONS

Ivanov A.N., Savelyeva M.S., Kurtukova M.O., Tyapkina D.A., Surmenev R.A., Surmeneva M.A., Parakhonskiy B.V., Shkarina S.N., Ul’yanov V.Y., Bugaeva I.O., Norkin I.A.