EMBRYONAL DEVELOPMENT OF THE PERITONEAL CONNECTIVE TISSUE OF ALBINO MICE (ELECTRON MICROSCOPIC STUDY)

Abstract

Objective - to study ultrastructural changes in the mesenchyme of the embryo participating in embryonic development of peritoneal connective tissue in mice. Material and methods. Mesenchymal cells and the structure of fibroblasts during the process of histogenesis of the connective tissue of the peritoneum were studied by electron microscopy and morphometry in 25 mice embryos on the 13th and 21st days of intrauterine development. Results. On the 13th day of mice embryogenesis, the submesothelial tissue was represented by mesenchyme, the differentiation of the cells of which was accompanied by their nonspecific changes and the appearance of numerous mitoses. On the 15th day of embryogenesis and onward, the size and quantity of organelles responsible for collagen synthesis in mesenchymal cells increased, and secretory vesicles appeared in the rough endoplasmic reticulum. Single collagen fibrils were found in submesothelial tissue. Conclusions. In the process of embryonic development of the peritoneal connective tissue in mice, collagen biosynthesis begins in the rough endoplasmic reticulum of mesenchymal cells as a result of the appearance of secretory vesicles containing protocollagen fibrils. The observed changes manifesting in the form of appearing numerous secretory vacuoles and protocollagen fibrils in the fibroblasts cytoplasm, plasma membrane destruction and the loss of significant portions of them, are indicative of holocrine secretion of collagen fibrils.

Keywords

peritoneum / connective tissue / ultrastructure / embryonic development

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RIGHTS & PERMISSIONS

Ivanova V.F., Kostyukevich S.V.