COMPLEX ASSESSMENT OF THE ADRENAL MEDULLA RESPONSE TO PARTIAL LASER DESTRUCTION OF A PAIRED ORGAN USING THE METHOD OF NATURAL PERIODIZATION OF BIOLOGICAL PROCESSES

Abstract

Objective - complex assessment of morphological changes in the adrenal medulla (AM) after partial laser destruction of a paired organ, based on the calculation of variation coefficients and the identification of natural periods Material and methods. On sections of the adrenal glands of adult rats (n=41), with a differentiating staining of adrenalocytes and noradrenalocytes, morphometry of 19 AM parameters was performed during 2 months after a surgical laser injury to the paired organ. The data were processed according to the natural periodization method. Results. According to the degree of variation of the parameters in each time point from their average values for the entire observation period and from control we calculated the coefficients of variation Cv and Cvc. Their dynamics allowed us to reveal the natural periods: I period - enhanced response (1-14 days) with phases I.1 - a quick, but weaker reaction (days 1-7) and I.2 - a slow, but stronger reaction (7-14 days); and II period - normalization (days 14-56). Passing the curves of Cvc and Cv through the time point of the 21st day almost in a straight-line indicates a possibility of optimizing similar experiments taking into account this time point. The convergence of all parameters with the control on the 56th day shows that it is impractical to prolong the experiment. Conclusions. 1) The crucial point of the systemic reaction of AM as a paired structure to laser damage is the 14th day. 2) When planning similar experiments, it is possible to optimize them taking into account the 21st day. 3) Extension of similar experiments longer than 2 months is not rational.

Keywords

adrenal medulla / natural periodization of biological processes / coefficient of variation / laser damage / systemic and compensatory-adaptive reactions

References

[1] Автандилов Г. Г., Яблучанский Н. И., Губенко В. Г. Системная стереометрия в изучении патологического процесса. М.: Медицина, 1981.

[2] Гейниц А. В., Цыганова В. И. Лазерные технологии в медицине: настоящее и будущее // Лазерная медицина. 2014. Т. 18, вып. 4. С. 11-12.

[3] Гуцол А. А., Кондратьев Б. Ю. Практическая морфометрия органов и тканей. Томск: Изд-во Томск. ун-та, 1988.

[4] Катинас Г. С., Быков В. Л. Метод естественной периодизации процессов // Арх. анат. 1976. Т. 71, вып. 9. С. 98-103.

[5] Кемоклидзе К. Г. Модификация метода естественной периодизации биологических процессов // Морфология. 2016. Т. 150, вып. 4. С. 80-83.

[6] Кемоклидзе К. Г., Тюмина Н. А. Динамика количественных показателей мозгового вещества надпочечника крысы после частичной лазерной деструкции // Морфология. 2014. Т. 46, вып. 6. С. 63-67.

[7] Лакин Г. Ф. Биометрия. М.: Высшая школа, 1990.

[8] Трухачева Н. В. Математическая статистика в медико-биологических исследованиях с применением пакета Statistica. М.: ГЭОТАР-Медиа, 2012.

[9] Honore L. H. A light microscopic method for the differentiation of noradrenaline and adrenaline producing cells of the rat adrenal medulla // J. Histochem. and Cytochem. 1971. Vol. 19, № 8. P. 483-486.

[10] Welch A. J., van Gemert M. J. C. Overview of optical and thermal laser-tissue interaction and nomenclature. In book: Optical-thermal response of laser-irradiated tissue. Dordrecht : Springer, 2011. Ch. 1. P. 3-11.

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Kemoklidze K.G.