ALTERATION OF NEUTROPHILIC GRANULOCYTES AND LYMPHOCYTES UNDER THE INFLUENCE OF QUANTUM DOTS (AN ATOMIC FORCE MICROSCOPIC STUDY)

Abstract

Objective - to analyze the main parameters of neutrophilic granulocytes and lymphocytes to assess the extent of their alteration by quantum dots with different coatings and to study the main types of cell death during this interaction. Material and methods. Neutrophilic granulocytes and lymphocytes from blood of 31 healthy donors of both sexes aged from 20 to 47 years were studied after exposure to three types of quantum dots with different coating. The main morphometric characteristics and morphological features of different types of cell death were assessed by atomic force microscopy. Results. The quantum dots in the CL50 cause alteration of leukocytes fraction, which results in an increase in height, a decrease in the adhesion area and coefficient in neutrophilic granulocytes, a decrease in height and an increase in the area of adhesion in lymphocytes. Conclusion: Under the influence of quantum dots, regardless of the type of their coating, similar mechanisms of death in neutrophilic granulocytes and lymphocytes were observed: necrosis, apoptosis, ETosis, autophagy. In addition, a unique death mechanism, mummification, was found in neutrophilic granulocytes.

Keywords

neutrophilic granulocyte / lymphocyte / atomic force microscopy / cell death / quantum dots

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RIGHTS & PERMISSIONS

Pleskova S.N., Bobyk S.Z., Fomichev O.I., Kryukov R.N.