ASCORBIC ACID EFFECTS ON THE MORPHOMETRIC PARAMETERS OF NEURONS IN THE MOTOR CORTEX AND THE CAUDATE NUCLEUS OF GUINEA PIGS AFTER MODELING A SURGICAL ABDOMINAL WALL WOUND

Abstract

Objective - to study the effect of ascorbic acid (AA) on the structure of the caudate nucleus and motor cortex in guinea pigs after modeling of the abdominal wall wound. Material and methods. The work was performed on histological sections of brain tissue obtained from the area of the caudate nucleus and motor cortex of 13 guinea pigs after 14 days of subcutaneous administration of a 5 % ascorbic acid solution and modeling of an abdominal wall wound. The sizes of neuronal nuclei and perikarya, the density of neurons, the area and the density of blood vessels were estimated on the paraffin sections stained with cresyl violet acetate. Results. Treatment with AA led to an increase in the area of blood vessels in the caudate nucleus and to an increase in the size of neuronal perikarya in different cytoarchitectonic layers of the motor cortex. Modeling of the wound process led to the reduction of metabolic activity of neurons in the studied structures and to the deterioration of blood supply in the caudate nucleus. Treatment of animals with AA within 7 days before and 7 days after the operation was not able to ameliorate these effects completely. Conclusions. The study demonstrated that subcutaneous administration of a 5 % solution of ascorbic acid to a certain extent helps to increase the metabolic activity of motor cortex neurons and to improve the blood supply to the caudate nucleus in guinea pigs.

Keywords

motor cortex / caudate nucleus / size of perikarya and neuronal nuclei / area of blood vessels / ascorbic acid

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RIGHTS & PERMISSIONS

Nikishina N.A., Ishunina T.A.