THE STUDY OF THE EFFECT OF STRESS ON THE MORPHOLOGICAL CHANGES IN THE HIPPOCAMPUS AFTER SURGICAL INTERVENTIONS IN THE NASAL REGION

Abstract

Objective - to study the morphological changes in the hippocampus of the rat brain following modeling septoplasty. Material and methods. The study was conducted on 80 adult male rats. In the experimental groups1 and 2, premedica tion with Phenazepamum was carried out. Group 1 included 30 rats subjected to local infiltration anesthesia with 2 % lidocaine solution; group 2 included 30 rats subjected to local infiltration anesthesia with 2 % solution of articain & epinephrine (0,5 mg/100 ml) and postoperative analgesia with diclofenac sodium solution for 6 days after surgery. Groups 3 and 4 were controls and included 10 animals each. In groups 1-3, fixation of the brain was performed prior to trepanation; in group 4, similar fixation was not performed and artifact dark neurons were counted. The number of dark neurons in the hippocampus was counted on hematoxylin-eosin stained brain sections on days 2, 6 and 14 after surgery. Results. In group 2, in the CA1, CA2, CA3 and DG zones, there were fewer dark neurons (DN) on day 6 compared with group 1. On 14th day, the number of DN in areas CA1 and CA2 was comparable in groups 2 and 3. In group 4, compared with group 3, the number of DN was greater in all studied hippocampal zones. Conclusion. The quantitative changes of DN in different zones of the hippocampus may indicate the severity of surgical stress following simulation of septoplasty under different types of anesthesia.

Keywords

hippocampus / dark neurons / rats / septoplasty / surgery stress

References

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RIGHTS & PERMISSIONS

Kastyro I.V., Kostyaeva M.G., Torshin V.I., Eremina I.Z., Ermakova N.V., Khamidulin G.V., Fatkhudinov T.K., Guschina Y.S., Surovtsev V.V., Drozdova G.A.