THE STUDY OF THE DYNAMICS OF THE MEDIATOR CONTENT OF SYMPATHETIC NEURONS IN EARLY ONTOGENESIS OF CAT

Abstract

Objective - to demonstrate the localization and immunohistochemical characteristics of cat sympathetic neurons in the superior cervical (SCG) and stellate (SG) ganglia. Material and methods. The work was performed on 1-, 10-, 20-, 30-day-old cats using retrograde axonal transport of Fast Blue (FB) which was injected in the neck muscle, and immunohistochemical methods. Results. FB-labeled neurons were detected in both studied ganglia in cats from the moment of birth. The largest number of labeled neurons in kittens of all age groups were immunoreactive (IR) to tyrosine hydroxylase (TG). The percentage of labeled neurons reactive both to TG and neuropeptide Y significantly increased in both ganglia during the first 10 days of life. The percentage of calbindin-IR neurons in the SCG decreased from birth by the 30th day of life, in the SG - increased in the first 10 days and then decreased by the 30th day of life. Immunoreactivity to choline acetyltransferase, vasointestinal polypeptide, and neuronal nitric oxide synthase were found in less than 1 % of labeled neurons in kittens of all age groups. Somatostatin-IR labeled neurons were not detected in the SCG and SG. Conclusions. In ontogenesis a reorganization of the mediator content of sympathetic neurons occurs, which in cats ends by the 20th day of life.

Keywords

Autonomic nervous system / sympathetic ganglia / ontogenesis

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Emanuilov A.I.