MORPHOLOGICAL AND NEUROCHEMICAL CHARACTERISTICS OF THE SUBICULAR COMPLEX OF THE HUMAN FETAL BRAIN

Abstract

Objective - to study the characteristics of cytoarchitecture and chemoarchitecture of the subicular complex in the human fetal brain. Material and methods. Ten hemispheres of human fetal brain were studied at 20-26 weeks of gestation. The material was obtained from the archive of the Department of Pathological Anatomy with a Course of Forensic Medicine of St. Petersburg State Pediatric Medical University. Histological paraffin sections were stained according to Nissl method, and immunohistochemically with antibodies raised against the layer-specific proteins SATB2, FOXP1, CTIP2, TBR1. Results. The deep layers (py, po, V, VI) of the subicular complex, which includes subiculum, presubiculum and parasubiculum, contained FOXP1 / CTIP2 and TBR1 immunopositive neurons, and the superficial layers (II, III) contained SATB2positive cells. Conclusions. Subiculum demonstrates the features of two cortical formations: the allocortex (the layer of FOXP1+/CTIP2+ cells, the absence of lamina dissecans) and the periallocortex (the layer of TBR1+ neurons). Presubiculum and parasubiculum are components of the periallocortex, which is indicated by the island pattern of SATB2+ layers II, III and the presence of lamina dissecans.

Keywords

subiculum / presubiculum / parasubiculum / layer-specific proteins

References

[1] Ding S. L. Comparative anatomy of the prosubiculum, subiculum, presubiculum, postsubiculum and parasubiculum in human, monkey, and rodent // J. Comp. Neurol. 2013. Vol. 521, № 18. P. 4145-4162. doi: 10.1002/cne.23416

[2] Herculano-Houzel S., Lent R. Isotropic fractionator: a simple, rapid method for the quantification of total cell and neuron numbers in the brain // J. Neurosci. 2005. Vol. 25, № 10. P. 2518- 2521. doi: 10.1523/JNEUROSCI.4526-04.2005

[3] Insausti R., Muñoz-López M., Insausti A. M., Artacho-Pérula E. The Human Periallocortex: Layer Pattern in Presubiculum, Parasubiculum and Entorhinal Cortex. A Review // Front. Neuroanat. 2017. Vol. 11. P. 84. doi: 10.3389/fnana.2017.00084

[4] Kang D. W., Lim H. K., Joo S. H., Lee N. R., Lee C. U. The association between hippocampal subfield volumes and education in cognitively normal older adults and amnestic mild cognitive impairment patients // Neuropsychiatr. Dis. Treat. 2018. Vol. 14. Р. 143-152. doi: 10.2147/NDT.S151659

[5] Saito T., Hanai S., Takashima S., Nakagawa E., Okazaki S., Inoue T., Miyata R., Hoshino K., Akashi T., Sasaki M., Goto Y., Hayashi M., Itoh M. Neocortical layer formation of human developing brains and lissencephalies: consideration of layer-specific marker expression // Cereb. Cortex. 2011. Vol. 21, № 3. P. 588- 596. doi: 10.1093/cercor/bhq125

RIGHTS & PERMISSIONS

Krasnoschekova E.I., Zykin P.A., Tkachenko L.A., Baskakov A.V., Nasyrov R.A.