SPATIAL ORGANIZATION OF THE DORSAL RAPHE NUCLEUS PROJECTIONS TO FUNCTIONALLY DIFFERENT REGIONS OF THE BASAL NUCLEI IN DOG BRAIN

Abstract

Objective - to study the projections from the individual subregions of the dorsal raphe nucleus (DRN) to the functionally different regions of the basal nuclei of dog forebrain. Material and methods. Using the method based on the retrograde axonal transport of the horseradish peroxidase (HRP), which was injected in the functionally different regions of the basal nuclei of the 43 outbred dogs, the spatial organization of the projections to these structures from different parts of the DRN was studied. Forty-eight hours after cerebral perfusion fixation, HRP was histochemically revealed in the DRN neurons on the serial coronal brain sections using tetramethylbenzidine. The number of the labeled neurons was counted under a microscope in each of studied parts, which were identified on the serial coronal toluidine- stained sections of celloidin-embedded brain. Results. The spatial organization of the projections to the functionally different segments of the basal nuclei from the individual functionally different parts of the DRN was studied in dogs by the method based on the retrograde axonal transport of HRP. The peculiarities of the topography of projection connections were revealed, indicating the possibility of segregated conduction of information from the dorsal part of the DRN, which has connections with the limbic structures, to the limbic subdomains of striatum. Observed in the entopeduncular nucleus, ventral pallidum, deep mesencephalic nucleus and medial portion of pedunculopontine nucleus overlapping of the terminal fields of neurons from all parts of DRN, receiving and transmitting functionally different information to the basal nuclei and associated structures, indicate its possible integration in these nuclei. In fact, the structural basis of information processing in the morpho-functional system of basal nuclei was analyzed. Conclusions. The presented data on the topographic organization of the projections directed to the functionally different regions of basal nuclei from various parts of DRN indicate the possibility of the influence of different parts of DRN on a wide range of behavioral and physiological processes in which basal nuclei are involved. The revealed system of connections participates in carrying the information, its integration in the morpho-functional system of basal nuclei, and serves as a structural basis for understanding of the mechanisms of their functioning in normal and pathological conditions.

Keywords

basal nuclei / deep mesencephalic nucleus / zona incerta / pedunculopontine nucleus / striatum

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RIGHTS & PERMISSIONS

Gorbachevskaya A.I.