THE PROLIFERATION ACTIVITY IN RAT OLFACTORY BULB IN A NEURODEGENERATION MODEL

Abstract

Objective - to evaluate the effect of neuronal death in the neonatal period on the proliferative activity in the rat olfactory bulb (OB). Material and methods. The study was conducted on 48 male Wistar 1 to 90 days of age. The object of study was the right OB. Neurodegeneration was induced by single subcutaneous injection of 10 % capsaicin solution to the rats on the first day of life. Ki-67 was detected using monoclonal rabbit antibodies (ab16667, UK, 1:100). Microphotographs of standard sections were used to determine the numerical density of positive nuclei (units/mm2). Results. The activity of cell proliferation in OB depended on the age and layer. In response to capsaicin administration, the proliferative activity increased during the first week. The intensity of the activation had topological characteristic - the maximum and earliest effect was observed in the center of the OB and then it spreaded to the outer layers. At the beginning of the observation, the main source of proliferating cells was the germinative zone of the OB, in the subsequent periods - the migrating neuronal precursors of the rostral migratory stream. Conclusion. Diffuse death of a part of neurons caused by the capsaicin administration leads to the development of a compensatory process manifested by the activation of the canonical stem niches and the germinative zone of the OB, as well as the prolongation of the proliferative activity of radially migrating cells to the 90th day of life in rats.

Keywords

Ki-67 / olfactory bulbs / neurogenesis / Ki-67 / ontogenesis / capsaicin

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RIGHTS & PERMISSIONS

Varentsov V.E., Rumyantseva T.A., Kiseleva E.V., Myasishcheva T.S.