IMMUNOHISTOCHEMICAL CHARACTERISTICS OF THE NEURONS OF RAT VENTROMEDIAL AND DORSOMEDIAL HYPOTHALAMIC NUCLEUS

Abstract

Objective - to identify the localization, percentage and morphometric characteristics of neurons immunoreactive (IR) to neuronal nitric oxide synthase (nNOS) and calbindin (CB) in the ventromedial (VMH) and dorsomedial (DMH) rat hypothalamic nuclei. Material and methods. The work was performed on female Wistar rats aged 3-4 months using immunohistochemical methods. Results. nNOS-IR and CB-IR neurons were detected in the VMH and DMH. VMH and DMH had heterogeneous neurochemical composition. The largest percentage of intensely fluorescent CB-IR neurons was demonstrated in DMH in the ventral region of the nucleus, while in the VMH CB-IR neurons with weak fluorescence were detected in large numbers in the ventrolateral region. In all areas of DMH and VMH, nNOS-IR neurons with low fluorescence intensity were demonstrated. The average cross-sectional area of CB-IR and nNOS-IR cells in the VMH and DMH was not significantly different from immunonegative neurons. Conclusions. The neurons of the VMH and DMH hypothalamic nuclei, which are involved in the regulation of metabolism in the body, contain nNOS and CB. In turn, VMH and DMH have heterogeneous neurochemical composition.

Keywords

hypothalamus / ventromedial nucleus / dorsomedial nucleus / neuronal nitric oxide synthase / calbindin

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RIGHTS & PERMISSIONS

Moiseev K.Y., Emanuylov A.I., Maslyukov P.M.