Reduction of Toxic Effect of Methyl Methacrylate on Human Cells in Culture Containing Sodium Hypochlorit

Abstract

The effect of sodium hypochlorite (SH) upon toxic response of cultured human fibroblasts to methyl methacrylate (MMA) which is a part of bone cement «Polacris» has been studied. Toxic effect of MMA in clinically adequate concentrations demonstrated dose-dependant suppression of total activity of mitochondrial enzymes and that was one of the showings of stress at the cellular level. MMA:SH mixture in volume ratio 1:1 and 10:1 caused reliable reduction of toxic effect on cultured cells as compared with pure MMA of the similar concentration. On the contrary, MMA: SH mixture in volume ratio 1:10 resulted in true decrease of fibroblasts viability as compared with the state of cells in presence of pure MMA. Thus it was shown that chemical experimental in vitro inactivation of MMA could be achieved only when MMA concentration was either higher or equal to SA concentration. That provided the reduction of metabolic disturbances in cells.

References

[1] Еропкин М.Ю. //Токсикол. вестн. - 1999. - N 5. - С. 7-13.

[2] Корнилов Н.В., Войтович А.В., Машков В.М., Эпштейн Г.Г. Хирургическое лечение дегенеративно-дистрофических поражений тазобедренного сустава. - СПб, 1997.

[3] Корнилов Н.В., Еропкина Е.М., Еропкин М.Ю. и др. //Травматол. ортопед. России. - 2000. -- N 2-3. - С. 25-29.

[4] Ребров А.П., Пономарева Е.Ю., Головачева Л.Ю. Применение раствора гипохлорита натрия при лечении больных пневмонией и хроническими неспецифическими заболеваниями легких: Метод, рекомендации. - Саратов, 1995.

[5] Bolls М., Fentem J.H. //ATLA. - 1992. - Vol. 20. - P. 368-389.

[6] Byrick R.J. //Can. J. Anaesth. - 1997. - Vol. 44, N 2. - P. 107-111.

[7] Clemedson C, Barile F.A., Ekwall B. et al. //ATLA. - 1998. - Vol. 26, Suppl. 1. - P. 93-129.

[8] Dahl O.E. //Acta Orthoped. Scand. - 1997. - Vol. 68, N6. - P. 607-614.

[9] Mosmann T. //J. Immunol. Meth. - 1983. - Vol. 65. - P. 55-63.

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