Dynamics of pertussis antibodies in the saliva of children revaccinated with DPT vaccine

Abstract

One of the promising methods that can be used in the rapid diagnosis of pertussis is the latex agglutination reaction (RLA), which we developed based on the platelet stained latex microagglutination reaction (RPOLMA). The aim of this work was to investigate the possibility of determining pertussis antibodies in the saliva of children revaccinated with DPT-vaccine by RPLMA. Polystyrene monodisperse latex with particle diameter of 0.55 μm, prepared in the All-Russian Research Institute of Synthetic Rubber (Leningrad) and sensitized with pertussis bacteria disintegrator containing 775 μg/ml protein (according to Lowry) in the ratio 1 : 2, was used as antigen for RPOLMA.

Keywords

Kazan Medical archive

References

[1] Амфитеатрова Н. Ф., Булатов H. М., Савинова А. И., Низамова Н. /О.//Казанский мед. ж.— 1987.—№ 4 - С. 308—311.

[2] Сигаева Л. А., Кузнецова Л. С., Окиншевич Е. А.//Журн. микробиол.— 1986.— № 3.— С. 43—47.

[3] Справочник по микробиологическим и вирусологическим методам исследования.//Под ред. М. О. Биргер.- М., 1982.

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