The effect of chronic energy drink consumption on cognitive and behavioral responses in laboratory rodents

Abstract

BACKGROUND: Energy drinks formulated to enhance mental and physical performance have been shown to induce dependence and withdrawal symptoms.

AIM: The study aimed to investigate the effects of chronic energy drink consumption on cognitive function and behavior in laboratory rodents.

METHODS: The study examined the effects of energy drinks (containing 30 mg and 200 mg of caffeine per 100 mL) administered intragastrically (0.5 mL for rats and 0.2 mL for mice) for 28 days. A total of 72 outbred SD male rats were tested using the Passive Avoidance Conditioning test and the Extrapolation Escape test, whereas 72 outbred CD1 male mice were evaluated in the Open Field and Social Behavior tests. Control animals received water in equivalent volumes, whereas experimental groups were administered energy drinks with varying caffeine concentrations. The statistical analysis was performed using SPSS (version 17.0) software, applying the Kolmogorov–Smirnov test, Wilcoxon signed-rank test, and Mann–Whitney U test. Results were considered statistically significant at p ≤ 0.05.

RESULTS: The low-caffeine energy drink impaired memory retention in the Passive Avoidance Conditioning test on days 21 and 28 (p = 0.045; p = 0.050, respectively) and increased response latency in the Extrapolation Escape test at later observation periods due to delayed motor activity, diving latency, and grid detection latency on day 14 (p = 0.002), day 21 (p = 0.003; p = 0.002; p = 0.002, respectively), and day 28 (p = 0.004; p = 0.002; p = 0.002, respectively). The high-caffeine energy drink reduced conditioned reflex formation in the Passive Avoidance Conditioning test on days 14, 21, and 28 (p = 0.050; p = 0.016; p = 0.009, respectively) and prolonged decision-making in the hierarchical task on days 14–28, increasing the latency for motor activity (p = 0.002), diving (p = 0.002), and grid detection (p = 0.002). Exploratory behavior in mice followed a wave-like pattern under the influence of energy drinks. Consumption of the low-caffeine energy drink was associated with increased horizontal activity and burrowing behavior during the early observation period (days 7–14, p = 0.002), followed by a decline at later stages (days 21–28, horizontal activity, p = 0.040; burrowing reflex, p = 0.009). A similar trend in horizontal activity was observed with the high-caffeine energy drink (days 7–28, p = 0.002). However, burrowing behavior was significantly reduced on days 21–28 (p = 0.003, p = 0.002, respectively). Increased anxiety reflected by prolonged freezing behavior was observed in mice consuming the high-caffeine energy drink on days 14–28 (p = 0.002; p = 0.002; p = 0.001, respectively). Frequent but brief grooming in mice consuming the low-caffeine energy drink also indicated heightened anxiety (day 14, p = 0.003; day 28, p = 0.005). Increased aggression was recorded in mice exposed to the low-caffeine energy drink on days 14–28 (p = 0.045; p = 0.002; p = 0.002, respectively), whereas persistent aggression was observed throughout the entire observation period in the high-caffeine energy drink group (p = 0.011; p = 0.002; p = 0.002; p = 0.002, respectively).

CONCLUSION: Chronic consumption of energy drinks (30 mg and 200 mg of caffeine per 100 mL for 28 days) does not enhance cognitive abilities and leads to impaired behavioral responses in rodents.

Keywords

energy drinks / rats / cognitive functions / behavior

References

[1] Gubareva DA. Experience study of Krasnodar students attidude to power drinks consumption. Medical news of North Caucasus. 2014;9(1):67–70. (In Russ.) doi: 10.14300/mnnc.2014.09019

[2] Губарева Д.А. Опыт изучения позиции учащейся молодежи города Краснодара в отношении потребления энергетических напитков // Медицинский вестник Северного Кавказа. 2014. Т. 9, № 1. С. 67–70. doi: 10.14300/mnnc.2014.09019

[3] Shcherbakova VA, Melikhova EP. Analyzing the composition of energy drinks and the effect that they can have on students. Russian Bulletin of Hygiene. 2022;(2):42–45. doi: 10.24075/rbh.2022.041.41

[4] Щербакова В.А., Мелихова Е.П. Анализ состава энергетических напитков и их влияние на здоровье студенческой молодежи // Российский вестник гигиены. 2022. № 2. С. 42–45. doi: 10.24075/rbh.2022.041

[5] Trofimov NS, Kutya SA, Kriventsov MA, et al. Energy drinks and their impact to the human health. Crimean Journal of Experimental and Clinical Medicine. 2019;9(3):75–82.

[6] Трофимов Н.С., Кутя С.А., Кривенцов М.А., и др. Влияние энергетических напитков на здоровье человека // Крымский журнал экспериментальной и клинической медицины. 2019. Т. 9, № 3. С. 75–82.

[7] Sekula M, Kaleta N, Pawlucki P, et al. Energy drinks – product ingredients and their potential toxicity. Journal of Education, Health and Sport. 2023;44(1):185–202. doi: 10.12775/JEHS.2023.44.01.012

[8] Sekula M., Kaleta N., Pawlucki P., et al. Energy drinks – product ingredients and their potential toxicity // Journal of Education, Health and Sport. 2023. Vol. 44, N. 1. P. 185–202. doi: 10.12775/JEHS.2023.44.01.012

[9] Burrows T, Pursey K, Neve M, Stanwell P. What are the health implications associated with the consumption of energy drinks? Nutr. Rev. 2013;71(3):135–48. doi: 10.1111/nure.12005

[10] Burrows T., Pursey K., Neve M., Stanwell P. What are the health implications associated with the consumption of energy drinks? // Nutr Rev. 2013. Vol. 71, N. 3. P. 135–48. doi: 10.1111/nure.12005

[11] Anuchin AM, Youvs GG. Comparative analysis of ergogenic efficacy of energy drinks components (caffeine and bitter orange extract) in combination with alcohol. Problems of Nutrition. 2014;(1):61–66. (In Russ.)

[12] Анучин А.М., Ювс Г.А. Сравнительный анализ эффективности действия эргогенных компонентов энергетических напитков (кофеина и экстракта горького апельсина) в сочетании с алкоголем // Вопр. питания. 2014. № 1. С. 61–66.

[13] Zenkova EA. Experimental study of the possible development of withdrawal syndrome after prolonged use of energy drinks. Perm Medical Journal. 2019;36(2):102–107. doi: 10.17816/pmj362102-107

[14] Зенкова Е.А. Экспериментальное изучение возможного развития абстинентного синдрома после длительного применения энергетических напитков // Пермский медицинский журнал 2019. Т. 36, № 2. С. 102–107. doi: 10.17816/pmj362102-107

[15] Zastrozhin MS, Drozhzhina NA. Epidemiologic aspects of energy drink intake in Russian Federation. Problems of Nutrition. 2015;84(2):19–24. (In Russ.)

[16] Застрожин М.С., Дрожжина Н.А. Эпидемиологические аспекты потребления энергетических напитков на территории Российской Федерации // Вопр. питания. 2015. № 2. С. 19–24.

[17] Abdullaev FZ, Babaev NM, Shikhieva LS. Features of coronary artery patterns and percutaneous coronary intervention in patients with acute coronary syndrome and stable angina in patients aged below 40 years. Kazan medical journal. 2020;101(1):8–24. doi: 10.17816/KMJ2020-18

[18] Абдуллаев Ф.З., Бабаев Н.М., Шихиева Л.С. Особенности поражения венечных артерий и эндоваскулярной реваскуляризации миокарда при остром коронарном синдроме и стабильной стенокардии у пациентов моложе 40 лет // Казанский мед. ж. 2020. Т. 101, № 1. C. 18–24. doi: 10.17816/KMJ2020-18

[19] Reis R, Charehsaz M, Sipahi H, et al. Energy drink induced lipid peroxidation and oxidative damage in rat liver and brain when used alone or combined with alcohol. J Food Sci. 2017;82(4):1037–1043. doi: 10.1111/1750-3841.13662

[20] Reis R., Charehsaz M., Sipahi H., et al. Energy drink induced lipid peroxidation and oxidative damage in rat liver and brain when used alone or combined with alcohol // J Food Sci. 2017. Vol. 82, N. 4. P. 1037–1043. doi: 10.1111/1750-3841.13662

[21] Ugwuja E. Biochemical effects of energy drinks alone or in combination with alcohol in normal albino rats. Adv Pharm Bull. 2014;4(1):69–74. doi: 10.5681/apb.2014.011

[22] Ugwuja E. Biochemical effects of energy drinks alone or in combination with alcohol in normal albino rats // Adv Pharm Bull. 2014. Vol. 4, N. 1. P. 69–74. doi: 10.5681/apb.2014.011

[23] Guidelines for conducting preclinical studies of medicinal products / ed. N.A. Mironov. Part one. Moscow: Grif i K, 2012. (In Russ.)

[24] Руководство по проведению доклинических исследований лекарственных средств / под ред. Н.А. Миронова. Часть первая. Москва: Гриф и К, 2012.

[25] Buresh Y, Bureshova O, Huston D. Methods and basic experiments in the study of brain and behavior / ed. A.S. Batueva. Moscow: Higher. school, 1991. (In Russ.)

[26] Буреш Я., Бурешова О., Хьюстон Д. Методики и основные эксперименты по изучению мозга и поведения / под ред. А.С. Батуева. Москва: Высш. шк., 1991.

[27] Tkachenko MO, Gubina-Vakylyuk GI, Gorbach TV, et al. Oral consumption of caffeinated energy drinks affects the morphofunctional state of stress-associated endocrine glands. The Journal of V.N. Karazin Kharkiv National University. 2018;(35):6–11. (In Russ.)

[28] Ткаченко М.А., Губина-Вакулик Г.И., Горбач Т.В., и др. Влияние перорального употребления энергетических напитков на морфофункциональное состояние стресс-организующих эндокринных желез // Вестник Харьковского национального университета имени В.Н. Каразина. 2018. № 35. С. 6–11.

[29] Tkachenko AS, Gubina-Vakulyck GI, Onishchenko AI, et al. Oral consumption of caffeinated energy drinks increases expression of KI-67 but decreases brain-derived neurotrophic factor in the brain of rats. Brunei International Medical Journal. 2018;14(1):140–146.

[30] Tkachenko A.S., Gubina-Vakulyck G.I., Onishchenko A.I., et al. Oral consumption of caffeinated energy drinks increases expression of KI-67 but decreases brain-derived neurotrophic factor in the brain of rats // Brunei International Medical Journal. 2018. Vol. 14, N. 1. P. 140–146.

[31] Tkachenko AS, Gubina-Vakulyck GI, Kauk OI, et al. Changes in S100 protein expression in the cerebellum of rats orally exposed to energy drinks. MJBMB. 2019;22(3):38–42.

[32] Tkachenko A.S., Gubina-Vakulyck G.I., Kauk O.I., et al. Changes in S100 protein expression in the cerebellum of rats orally exposed to energy drinks // MJBMB. 2019. Vol. 22, N. 3. P. 38–42.

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