Neuropeptide Y is involved in the regulation of the frequency and strength of rat atrium spontaneous contraction

Abstract

BACKGROUND: The relevance of the problem increases due to the established cardioprotective effect of neuropeptide Y in pathological conditions such as hypertension, heart failure, and its possible therapeutic use in cardiovascular diseases.

AIM: Study of the effect of neuropeptide Y on the frequency of spontaneous activity and amplitude-time parameters of the myocardium contraction of the rats’ right atrium during different periods of postnatal ontogenesis.

MATERIAL AND METHODS: The experiments used 73 white outbred rats of 7 days (n=23), 21 days (n=25) and 100 days (n=25) of age. Using the classical strain-gauge method, the parameters of isometric contraction of isolated rat atrial myocardium were determined with the addition of a non-selective Y-receptor agonist. The isometric contraction curve was processed using the Chart 8.0 program. Statistical processing of the results was performed in MS Excel and IBM SPSS Statistics 2020. The significance of the differences was calculated using the Student’s t test. Data were presented as mean ± error of mean (M±m); n was the number of atrial myocardium preparations used. Changes were considered statistically significant at p <0.05.

RESULTS: Neuropeptide Y at a concentration of 10^–7 M reduces the frequency of spontaneous activity of the right atrium strips of rats with a preserved sinus node in 7, 21 and 100-day-old animals by 8.1%, 15% (p=0.038) and 7.9% (p=0.046) respectively in comparison with the control value. The amplitude of isometric contraction of the atrial myocardium in 7-day-old animals decreases by 12.4% (p=0.041), the duration of contraction decreases in 21-day-old animals by 12% (p=0.040), and in 100-day-old animals does not change compared to control.

CONCLUSION: Neuropeptide Y reduces the frequency of spontaneous activity, increases the contraction duration, reduces the isometric contraction amplitude of rat myocardial strips in early postnatal ontogenesis.

Keywords

neuropeptide Y / spontaneous contraction frequency / rat / contractility / heart

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