Comprehensive assessment of certain trace elements, hormones and enzymes in patients with exogenous-constitutional obesity in predicting transition to metabolic syndrome

Abstract

Aim. To study the relationship between the trace element status (chromium, zinc and copper) and hormonal activity (insulin, leptin) in women with metabolically healthy and unhealthy obesity phenotypes, depending on the type and severity of obesity.

Methods. A cross-sectional study was conducted to compare 288 women with exogenous-constitutional obesity aged 25–45 years and healthy women of similar age (n=38). The comparative analysis was carried out in groups of patients with android and gynoid ECO types and varying degrees of obesity. The study was conducted in the NUZ “Department Clinical Hospital of the Kazan station of JSC Russian Railways” between 2016 and 2020. Along with clinical laboratory tests, the indicators of carbohydrate and lipid metabolism (including the determination of hormones), the activity of enzyme systems and trace element content were determined. The statistical significance of the differences was assessed by using the non-parametric Spearman's rank correlation test.

Results. It was revealed that the type of obesity associated with the indicators of lipid and carbohydrate metabolism, the activities of catalase and peroxidase, and trace element content. In patients with abdominal obesity, the concentration of copper was higher than in the comparison group, and chromium and zinc — lower than in patients with gynoid obesity and in the control group. In patients with android obesity, a high correlation was found between the serum copper level (r=0.98) and body fat percentage (r=0.74) compared with patients with gynoid type obesity. The correlation of chromium level with the level of blood glucose (r=0.58), triglycerides (r=0.66), cholesterol (r=0.60) and catalase enzyme activity (r=0.54) as well as correlation of zinc level with the level of blood glucose (r=0.74), cholesterol (r=0.77), triglycerides (r=0.90), catalase (r=0.57), and peroxidase (r=0.59) were revealed. Also, significant differences in the level of trace elements in patients with varying degrees of obesity were found.

Conclusion. An increase in copper concentration and a decrease in the concentration of chromium and zinc are unfavorable signs in obese patients associated with the activation of oxidative stress, hyperplastic processes and a high risk of developing a metabolic syndrome.

Keywords

microelements / exogenous-constitutional obesity / metabolic syndrome / activity of enzyme systems

References

[1] Lambadiari V., Korakas E., Tsimihodimos V. The impact of dietary glycemic index and glycemic load on postprandial lipid kinetics, dyslipidemia and cardiovascular risk. Nutrients. 2020; 12 (8): 2204. DOI: 10.3390/nu12082204.

[2] Mychka V.B., Vertkin A.L., Vardaev L.I., Druzhilov M.A., Ipatkin R.V., Kalinkin A.L., Kuznetsova I.V., Kuznetsova T.Yu., Mekhtiev S.N., Morgunov Yu.L., Mil­ler A.M., Mamedov M.N., Osipova I.V., Pushkar D.Yu., Tapilskaya N.I., Titarenko V.L., Chumakova G.A., Shchekotov V.V., Aganezova N.V., Ametov A.S., Antropova O.N., Balan V.E., Bogachev R.S., Demidova T.Yu., Drapkina O.M., Lutsevich O.E., Naumov A.V., Oganov R.G., Patsenko M.B., Pyrikova N.V., Sigal A.S., Salov I.A., Smetnik V.P., Tebloev K.I., Tolstov S.N., Ulrikh E.A., Fisun A.Ya., Yureneva S.V., Yashkov Yu.I. Experts’ consensus on the interdisciplinary approach towards the management, diagnostics, and treatment of patients with metabo­lic syndrome. Kardiovaskulyarnaya terapiya i profilaktika. 2013; 12 (6): 41–82. (In Russ.)

[3] Мычка В.Б., Верткин А.Л., Вардаев Л.И., Дружилов М.А., Ипаткин Р.В., Калинкин А.Л., Кузнецова И.В., Кузнецова Т.Ю., Мехтиев С.Н., Моргунов Ю.Л., Миллер А.М., Мамедов М.Н., Осипова И.В., Пушкарь Д.Ю., Тапильская Н.И., Титаренко В.Л., Чумакова Г.А., Щекотов В.В., Аганезова Н.В., Аметов А.С., Антропова О.Н., Балан В.Е., Богачёв Р.С., Демидова Т.Ю., Драпкина О.М., Луцевич О.Э., Наумов А.В., Оганов Р.Г., Паценко М.Б., Пырикова Н.В., Сигал А.С., Салов И.А., Сметник В.П., Теблоев К.И., Толстов С.Н., Ульрих Е.А., Фисун А.Я., Юренева С.В., Яшков Ю.И. Консенсус экспертов по междисциплинарному подходу к ведению, диагностике и лечению больных с метаболическим синдромом. Кардиоваск. терап. и профил. 2013; 12 (6): 41–82.

[4] Grundy S.M., Williams C., Vega G.L. Upper body fat predicts metabolic syndrome similarly in men and ­women. Eur. J. Clin. Invest. 2018; 48 (7): e12941. DOI: 10.1111/eci.12941.

[5] Neeland I.J., Ross R., Després J.P., Matsuzawa Y., Yamashita S., Shai I., Seidell J., Magni P., Santos R.D., Arsenault B., Cuevas A., Hu F.B., Griffin B., Zambon A., Barter P., Fruchart J.C., Eckel R.H. Visceral and ectopic fat, atherosclerosis, and cardiometabolic disease: a position statement. Lancet Diabetes Endocrinol. 2019; 7 (9): 715–725. DOI: 10.1016/S2213-8587(19)30084-1.

[6] Chumakova G.A., Kuznetsova T.Yu., Druzhilov M.A., Veselovskaya N.G. Visceral adiposity as a global factor of cardiovascular risk. Russian Journal of Cardiology. 2018; (5): 7–14. (In Russ.) DOI: 10.15829/1560-4071-2018-5-7-14.

[7] Чумакова Г.А., Кузнецова Т.Ю., Дружилов М.А., Веселовская Н.Г. Висцеральное ожирение как глобальный фактор сердечно-сосудистого риска. Рос. кардиол. ж. 2018; (5): 7–14. DOI: 10.15829/1560-4071-2018-5-7-14.

[8] Tinkov A.A., Ajsuvakova O.P., Skalnaya M.G., Skalny A.V. Relationship between serum metal and metalloid levels with metabolic risk markers in overweight and obese women. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii. 2020; 23 (5): 23–29. (In Russ.) DOI: 10.29296/25877313-2020-05-04.

[9] Тиньков А.А., Айсувакова О.П., Скальная М.Г., Скальный А.В. Взаимосвязь сывороточной концентрации металлов и металлоидов с маркёрами метаболического риска женщин с избыточным весом и ожирением. Вопр. биол., мед. и фармацевтич. химии. 2020; 23 (5): 23–29. DOI: 10.29296/25877313-2020-05-04.

[10] Shi Y., Zou Y., Shen Z., Xiong Y., Zhang W., Liu C., Chen S. Trace elements, PPARs, and metabolic syndrome. Int. J. Mol. Sci. 2020; 21 (7): 2612. DOI: 10.3390/ijms21072612.

[11] Korchina T.Yа., Korchin V.I., Lubyako E.A. Peculiarities of the elemental status in persons with metabolic syndrome living in the north (exemplified by Khanty-Mansiysk). Vestnik Severnogo (Arkticheskogo) federal'nogo universiteta. ­Seriya: Mediko-biologicheskie nauki. 2015; (4): 116–125. (In Russ.) DOI: 10.17238/issn2308-3174.2015.4.116.

[12] Корчина Т.Я., Корчин В.И., Лубяко Е.А. Особенности элементного статуса у лиц с метаболическим синдромом, проживающих в северном регионе (на примере г. Ханты-Мансийска). Вестн. Северного (Арктического) федерального ун-та. Серия: Медико-биологические науки. 2015; (4): 116–125. DOI: 10.17238/issn2308-3174.2015.4.116.

[13] Korchina T.Y., Korchin V.I., Sukhareva A.S., Safarova O.A., Cherepanova K.A., Bogdanovich A.B., Sha­rifov M.I., Nekhoroshikh S.S. Elemental status of adult non-indigenous population of Khanty-Mansi autonomous region. Ekologiya cheloveka. 2019; (10): 33–40. (In Russ.) DOI: 10.33396/1728-0869-2019-10-33-40.

[14] Корчина Т.Я., Корчин В.И., Сухарева А.С., Сафарова О.А., Черепанова К.А., Богданович А.Б., Шарифов М.И., Нехороших С.С. Элементный статус взрослых некоренных жителей Ханты-Мансийского автономного округа. Экология человека. 2019; (10): 33–40. DOI: 10.33396/1728-0869-2019-10-33-40.

[15] Amin M.N., Siddiqui S.A., Uddin M.G., Ibrahim M., Uddin S.M.N., Adnan M.T., Rahaman M.Z., Kar A., Islam M.S. Increased oxidative stress, altered trace elements, and macro-minerals are associated with female obesity. Biol. Trace Elem. Res. 2020; 197 (2): 384–393. DOI: 10.1007/s12011-019-02002-z.

[16] Kido T., Ishiwata K., Suka M., Yanagisawa H. Inflammatory response under zinc deficiency is exacerba­ted by dysfunction of the T helper type 2 lymphocyte-M2 macrophage pathway. Immunology. 2019; 156 (4): 356–372. DOI: 10.1111/imm.13033.

[17] Kozlovskaya A.V., Potolitsina N.N., Shadrina V.D., Boj­ko E.R. The vitamins and enzymes of the antioxidant system in blood of women in the North. Izvestiya Komi nauchnogo tsentra URO RAN. 2016; (1): 112–115. (In Russ.)

[18] Козловская А.В., Потолицына Н.Н., Шадрина В.Д., Бойко Е.Р. Витамины и ферменты антиоксидантной системы в крови у женщин на Севере. Извес­тия Коми науч. центра УрО РАН. 2016; (1): 112–115.

[19] Stenzel A.P., Carvalho R., Jesus P., Bull A., Pereira S., Saboya C., Ramalho A. Serum antioxidant associations with metabolic characteristics in metabolically healthy and unhealthy adolescents with severe obesity: An observational study. Nutrients. 2018; 10 (2): 150. DOI: 10.3390/nu10020150.

[20] World Health Organization (WHO). Obesity: preventing and managing the global epidemic. Report of a WHO Consultation on Obesity (3–5 June 1997). Geneva, Switzerland: World Health Organization. 1997. https://apps.who.int/iris/handle/10665/63854 (access date: 05.01.2021).

[21] Iskra R.Ja. The Effect of Different Le­vels of Chromium Chloride Supplementation on Antioxidant Defense System in the Rats. Journal of Siberian Fe­deral University. Biology. 2013; 6: 246–256. (In Russ.)

[22] Искра Р.Я. Влияние различных концентраций хлорида хрома на систему антиоксидантной защиты у крыс. Ж. Сибирского федерального ун-та. Биология. 2013; 6: 246–256.

[23] Husain N., Mahmood R. Copper (II) generates ROS and RNS, impairs antioxidant system and damages membrane and DNA in human blood cells. Environmental Sci. Pollution Res. Intern. 2019; 26: 20 654–20 668. DOI: 10.1007/s11356-019-05345-1.

[24] Hassan I., Ebaid H., Alhazza I.M., Al-Tamimi J., Aman S., Abdel-Mageed A.M. Copper mediates anti-inflam­matory and antifibrotic activity of Gleevec in hepatocellular carcinoma-induced male rats. Can. J. Gastroente­rol. Hepatol. 2019; 1029: 1–11. DOI: 10.1155/2019/9897315.

[25] Polloni L., Seni Silva A.C., Teixeira S.C., Azevedo F.V.P.V., Zóia M.A.P., da Silva M.S., Lima P.M.A.P., Correia L.I.V., do Couto Almeida J., da Silva C.V., Rodrigues Ávila V.M., Goulart L.R.F., Morelli S., Guerra W., Oliveira Júnior R.J. Action of copper (II) complex with β-diketone and 1,10-phenanthroline (CBP-01) on sarcoma cells and bio­logical effects under cell death. Biomed. Pharmacothe­rap. 2019; 112: 108586. DOI: 10.1016/j.biopha.2019.01.047.

[26] Tihova G.P. Planning clinical research. Question #1: How to calculate enough sample vo­lume? Regionarnaya anesteziya i lechenie ostroy boli. 2014; (3): 57–63. (In Russ.)

[27] Тихова Г.П. Планируем клиническое исследование. Вопрос №1: как определить необходимый ­объём выборки? Регионарная анестезия и лечение острой боли. 2014; (3): 57–63.

[28] Al‐Daghri N.M., Al-Attas O.S., Alokail M.S., Alkharfy K.M., Charalampidis P., Livadas S., Kollias A., Sabico S.L., Chrousos G.P. Visceral adiposity index is highly associated with adiponectin values and glycaemic disturbances. Eur. J. Clin. Invest. 2013; 43 (2): 183–189. DOI: 10.1111/eci.12030.

[29] Yang F., Wang G., Wang Z., Sun M., Cao M., Zhu Z., Fu Q., Mao J., Shi Y., Yang T. Visceral adiposity index may be a surrogate marker for the assessment of the effects of obesity on arterial stiffness. PLoS One. 2014; 9 (8): e104365. DOI: 10.1371/journal.pone.0104365.

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Nikishova T.V., Kurnikova I.A.